Skip to main content Skip to navigation

Methods reporting

Microscope (Make, Model) Andor, Revolution
Inverted or upright Inverted
Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any)

Select the one/s used:

Nikon, 100x, 1.45, oil,

Nikon, 60x, 1.40, oil,

Nikon, 20x, 0.75, air,

Light source(s)

Select the one/s used:

405 nm, 488 nm, 561 nm, 640 nm lasers

Filters, dichroic mirrors, beam splitters

Select the one/s used:

Dichroic: 405/488/561/640 nm or 405/488/591 nm

Emission filters:

DAPI - 452/45

GFP - 525/50

TRITC - 607/36

mCherry LP - 568 LP

Cy5 - 685/40

GFP/mCherry high speed - 440/521/607/700

Information on other filters are available on request. channel set-up
Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective

Nikon Ti-Eclipse

Yokogawa CSU X-10 spinning disk unit

Okolabs microscope incubator

Prior motorised xy stage

Prior 400 um z-piezo

Acquisition software and settings Andor iQ
Camera manufacturer, model Andor iXon 897