Skip to main content Skip to navigation

Methods reporting

Microscope (Make, Model) Andor, Revolution XD
Inverted or upright Inverted
Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any)

Select the one/s used:

Nikon, 100x, 1.45, oil,

Nikon, 40x, 1.30, oil,

Nikon, 20x, 0.75, air,

Nikon, 10x, 0.45, air,

Light source(s)

Select the one/s used:

457 nm, 488 nm, 515 nm, 561 nm lasers

Filters, dichroic mirrors, beam splitters

Select the one/s used:

Dichroic: 457/515/647 nm or 488/568 nm

Secondary dichroic (when using dual-camera): 561 nm longpass

Emission filters:

CFP - 480/40

GFP - 525/50

YFP - 545/40

RFP - 512/630

GFP TuCam - 514/30

RFP TuCam - 569 LP

Information on other filters are available on request. channel set-up
Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective

Nikon Ti-Eclipse

Yokogawa CSU X-10 spinning disk unit w/ Borealis

Andor FRAPPA photomanipulation unit

Andor MicroPoint laser ablation system

Okolabs microscope incubator

ASI motorised xy stage

ASI 400 um z-piezo

2x magnification

Acquisition software and settings Andor iQ
Camera manufacturer, model 2 x Andor iXon Ultra 888