FV3000 methods reporting
| Microscope (Make, Model) | Olympus IX 83 |
| Inverted or upright |
Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Olympus, 60x, 1.20, oil, Plan Apo VC Olympus, 40x, 1.00, oil, Plan Apo VC Olympus, 20x, 0.7, air, Plan Fluor Olympus, 10x, 0.3, air, Plan Apo VC |
| Light source(s) |
Select the one/s used: 405 nm, 488 nm, 561 nm, 640 nm lasers |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Dichroic: 405/488/561/640 nm Variable emission filters, check the metadata. Eyepiece widefield imaging: DAPI: U-FBNA: ex 470-495 dm 505 em 510-550 U-FGWA: ex 530-550 dm 570 em 575-625 |
| fpbase.org channel set-up | |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective | |
| Acquisition software and settings | FluoView |
| Detector manufacturer, model | 4 x Olympus Multi-alkali PMT |