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FV3000 methods reporting

Microscope (Make, Model) Olympus IX 83
Inverted or upright


Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any)

Select the one/s used:

Olympus, 60x, 1.20, oil, Plan Apo VC

Olympus, 40x, 1.00, oil, Plan Apo VC

Olympus, 20x, 0.7, air, Plan Fluor

Olympus, 10x, 0.3, air, Plan Apo VC

Light source(s)

Select the one/s used:

405 nm, 488 nm, 561 nm, 640 nm lasers

Filters, dichroic mirrors, beam splitters

Select the one/s used:

Dichroic: 405/488/561/640 nm

Variable emission filters, check the metadata.

Eyepiece widefield imaging:


U-FBNA: ex 470-495 dm 505 em 510-550

U-FGWA: ex 530-550 dm 570 em 575-625 channel set-up  
Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective  
Acquisition software and settings FluoView
Detector manufacturer, model 4 x Olympus Multi-alkali PMT