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Methods reporting

Microscope (Make, Model) Nikon, Ti-Eclipse
Inverted or upright Inverted
Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any)

Select the one/s used:

Nikon, 100x, 1.40, oil, PlanApoVC

Nikon, 60x, 1.40, oil, PlanApoVC

Nikon, 40x, 0.75, air, PlanFluor

Nikon, 40x, 0.60, air, SPlanFluor

Nikon, 20x, 0.5, air, PlanFluor

Light source(s)

Nikon Intensilight

Filters, dichroic mirrors, beam splitters

Select the one/s used:

Filter sets:

DAPI: ex 360/40 dm 435 em 460/50

FITC: ex 480/30 dm 505 em 535/40

GFP LP: ex 470/40 dm 505 em 510LP

TRITC: ex 540/25 dm 565 em 605/55

CY-5: ex 635/30 dm 660 em 710/80

More filter information available on request. channel set-up
Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective

Okolabs microscope incubator

Okolabs Bold Line CO2 and temperature control

Nikon motorised xy stage

Prior motorised focus control

Sutter brightfield and fluorescence shutters

Acquisition software and settings NIS-Elements AR 4.20.06
Camera manufacturer, model Photometrics CoolSNAP MYO cooled CCD