Methods reporting
| Microscope (Make, Model) | Nikon, Ti-Eclipse |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Nikon, 100x, 1.40, oil, PlanApoVC Nikon, 60x, 1.40, oil, PlanApoVC Nikon, 40x, 0.75, air, PlanFluor Nikon, 40x, 0.60, air, SPlanFluor Nikon, 20x, 0.5, air, PlanFluor |
| Light source(s) |
Nikon Intensilight |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Filter sets: DAPI: ex 360/40 dm 435 em 460/50 FITC: ex 480/30 dm 505 em 535/40 GFP LP: ex 470/40 dm 505 em 510LP TRITC: ex 540/25 dm 565 em 605/55 CY-5: ex 635/30 dm 660 em 710/80 More filter information available on request. |
| fpbase.org channel set-up | https://www.fpbase.org/microscope/qsJjaPfreB6io2ffpwaLsV/ |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Okolabs microscope incubator Okolabs Bold Line CO2 and temperature control Nikon motorised xy stage Prior motorised focus control Sutter brightfield and fluorescence shutters |
| Acquisition software and settings | NIS-Elements AR 4.20.06 |
| Camera manufacturer, model | Photometrics CoolSNAP MYO cooled CCD |