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Methods reporting

Microscope (Make, Model) Nikon CSU-W1 spinning disk with SoRa upgrade
Inverted or upright Inverted
Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any)

Select the one/s used:

Nikon, 100x, 1.49, oil, CFI SR HP Apo TIRF

Nikon, 63x, 1.40, oil, CFI Plan Apo

Nikon, 40x, 1.30, oil, CFI Plan Fluor

Nikon, 20x, 0.5, air, CFI Plan Fluor

Light source(s)

Select the one/s used:

405 nm, 488 nm, 561 nm, 638 nm lasers

Filters, dichroic mirrors, beam splitters

Select the one/s used:

Dichroic: 405/488/561/640 nm

Secondary dichroic (when using dual-camera): 561 nm LP

Emission filters:

Blue - 446/60

Green - 525/50

Red - 600/52

FRed - 708/75

Information on other filters are available on request. channel set-up
Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective

Yokogawa CSU-W1 spinning disk unit with 50 um and SoRa disks

Nikon Perfect Focus autofocus

Okolab microscope incubator

Nikon motorised xy stage

Nikon 200 um z-piezo

Bruker MiniScanner photomanipulation unit

Acquisition software and settings NIS-Elements
Camera manufacturer, model 2 x Photometrics 95B Prime