The Q-Phase is a holographic microscope from Telight. The output is the phase image where the light delay (phase shift) across each part of the sample is stored as a quantitative value in the corresponding image pixels (Quantitative Phase Imaging). This allows for direct measurement of the cell dry mass distribution in real-time.

Q-phase is optimised for label-free live-cell imaging and automated cell culture analysis. It is also equiped with a widefield fluorescence modality. Q-phase is particularly suited to long-term live-cell imaging and has extremely low phototoxicity. Applications include cell morphology, cell death, cell cycle, and migration studies. The image acquisition software SophiQ performs cell segmentation and tracking and calculates parameters such as cell dry mass, density, area, growth speed, and euclidean distance.

SophiQ is also available on Dolly, our dedicated analysis machine.

Sample preparations is important for QPI - the media or buffer cannot have a meniscus. So it requires specific slides/dishes.
We have special lids for 35 mm dishes and 24-well-plates.

Located in MCBB room L1.22.

Please contact camdu@warwick.ac.uk or Maelle directly if you want to try this microscope.

Configuration still in construction, ask Maelle.

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Components

Objectives	4x air, 10x air, 20x air, 40x air, 60x oil
Light source	LED Lumencor Spectra for fluorescence, LED 660 nm for QPI.
Sample holders	1x microscopic slide, 2x 35mm dishes, 1 multi-well plate
Cameras	CMOS Allied Vision Alvium 1800 U-2040m , sCMOS Tucsen Dhyana 400BSI V3
Stage	Motorised xyz
Incubation	37 degrees, CO2
Filters	DAPI/FITC/TRITC, GFP/mCherry
Autofocus	Hardware-based autofocus

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