My research is focused on mass spectrometry based metabolomics and proteomics, their applications in areas including natural product drug discovery, drug metabolism, disease biomarker discovery and environmental analysis.
1. Bioactive natural product discovery from microorganism and plant, using a combination of liquid chromatography, high reoslution high accuracy MS, MS/MS, 1D and 2D NMR techniques, we carry out dereplication, identification, purification and structure elucidation of novel bioactive natural products.
2. Drug mechanism of action and metabolism analysis (ADME, pharmacokinetics), using stable isotope labeled compounds to investigate drug metabolism in both in vitro and in vivo systems, metabolites are identified through high resolution LC-MS/MS profiling, both the original drug molecues and their metabolites can be quantified in MRM mode on triple quadrupole mass spectrometer pharmacokinetic and pharmacodynamic evaluation.
3. Disease biomarker discovery and validation (for therapeutic and pharmacodynamics study) using comparative metabolomic and proteomic approach.
4. Environmental analysis of both organic and inorganic pollutants (agrochemicals such as pestcide, herbcides, veterinary antimicrobials, animal growth hormones,their leakage into the environment; human medicine and brekdown products entering the environment from waste water treatment plant) as well as environment originated antimicrobial resistance.
- L. Song, M. Jenner, J. Masschelein, C. Jones, M. Bull, S. Harris, R. Hartkoorn, A. Vocat, I. Romero-Canelón, P. Coupland, Paul, G. Webster, M. Dunn, R. Weiser, C. Paisey, S. Cole, J. Parkhill, E. Mahenthiralingam and G. L. Challis. Discovery and biosynthesis of gladiolin: a Burkholderia gladioli antibiotic with promising activity against Mycobacterium tuberculosis. J. Am. Chem. Soc. 2017, 139(23)7974-7981
O. Tsypik, R. Makitrynskyy, A. Bera, L. Song, W. Wohlleben, V. Fedorenko, B. Ostash, Role of GntR Family Regulatory Gene SCO1678 in Gluconate Metabolism in Streptomyces coelicolor M145, BioMed Research International, 2017, DOI.org/10.1155/2017/9529501
- Y. Inahashi, S. Zhou, M. Bibb, L. Song, M. Al-Bassam, M. Bibb and G. Challis. Watasemycin biosynthesis in Streptomyces venezuelae: thiazoline C-methylation by a type B radical-SAM methylase homologue. Chem. Sci. 2017, 8, 2823-2831