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Bryan Pietro Marzullo

Current Studies
PhD Project - FT-ICR MS

I am a PhD student in Peter O'Connor's group based in the Ion Cyclotron Resonance Laboratory, Millburn House, Department of Chemistry.
My PhD project is in collaboration with Syngenta and focused on "Detailed Structural Analysis of Agrochemicals using Advanced Mass Spectrometry Methods."

Structure elucidation is essential to ensure the correct target molecule has been synthesized and also supports the determination of any by-products and impurities. High-resolution accurate mass spectrometry has become the front-line tool for structural elucidation for agrochemicals and pharmaceuticals due to its ability to handle complex samples and characterize low-level components of interest. High mass accuracy and mass resolution allows reliable confirmation of elemental formula for precursor and product ions to interpret a spectrum and establish the structure. Elucidating the structure of a known molecule allows acquisition of a fingerprint spectrum of that molecule which can help in the elucidation of their respective metabolites. This is crucial for agrochemicals and drugs molecules, as their metabolites can be potentially toxic to humans, plants, and the environment.

Using different fragmentation techniques (such as collisionally activated dissociation (CAD), infrared multiphoton dissociation (IRMPD), electron induced dissociation (EID), and ultraviolet photodissociation (UVPD)) we investigate the fragmentation patterns of these species. We employ the advantages of two-dimensional mass spectrometry (2D MS) to aid with complex mixture samples; 2D MS does not require separation of complex mixtures prior to analysis and allows grouping of species that have similar core structures or similar functional groups.

Academic Background
M. Sc. (Distinction) in Analytical Science & Instrumentation - The University of Warwick (2016-2017)
B.Sc. (Hons) in Chemistry - The University of Manchester (2011 – 2014)
Previous projects
The University of Warwick - Master's Project
Master’s Project - Supercharging Polymeric Molecules via a Novel Supercharging Source & Structure Elucidation of Oxidative Debris

The aim of the project was to supercharge polymeric molecules using a home-built supercharging source and the structure elucidation of oxidative debris.

The supercharging source, developed at Warwick University, uses a superacid (CH5+) to supercharge the molecule.

The University of Manchester - Mini Projects
Synthesis: Preparation of TADDOL Ligands: Applications of Asymmetric Catalysis

Synthesising TADDOL, via a two-step Grignard reaction, to be used as a chiral catalyst, in a titanium complex form, for the synthesis of an enantiomerically pure product via an asymmetric Diels-Alder reaction.

Measurement & Analytical: Zeolites and Solid State Characterisation Techniques

Synthesise zeolites and Mobil Composition of Matter (MCM). Characterise them via X-Ray Diffraction, Small Angle X-Ray Scattering, Scanning Electron Microscopy and Atomic Force Microscopy.

Computational: Environmental Speciation

Producing a model to fit experimental data; using the model to propose a mechanism to prevent nuclear waste contamination of a stream.

Previous Experience
The University of Manchester, Chemical Engineering and Analytical Science

Analysing anti-bacterial coating on textiles via Solid Phase Micro-extraction Gas Chromatography-Mass Spectrometry and E-Nose.

The University of Manchester, Manchester Institute of Biotechnology

Dry work: Using COPASI to analyse the glycolysis mechanism in a yeast cell.
Investigate which enzyme(s) have the major effect on a single step in the model using the Metabolic Control Analysis (MCA) tool. Identify the flux at each step and minimised the flux for unwanted side reactions using information obtained from MCA.
Wet work: Ligation of yeast cells and DNA fragments for DNA molecular cloning.

University of Manchester, I.T. Department, Manchester Institute of Biotechnology

UPPCON 2018 - Leeds, UK (Poster Presentation)
BMSS (AIMS) 2018 - Syngenta, Bracknell, UK

Bryan Pietro Marzullo

Bryan Pietro Marzullo
in collaboration with Syngenta.
E-Mail: b dot marzullo at warwick dot ac dot uk