Prof Phillip Stansfeld

Phillip Stansfeld

Professor of Chemistry and Life Sciences

Phillip.Stansfeld@warwick.ac.uk
+44 (0)24 765 23864
Stansfeld GroupLink opens in a new window

About Me

Phillip is Professor in both the School of Life Sciences and the Department of Chemistry

Groups at Warwick

Dr Phillip StansfeldLink opens in a new windowLink opens in a new window

Affiliations

School of Life Sciences

Biography

2003 Bsc with Hons (1st Class) Biological Sciences, University of Edinburgh
2007 PhD Biochemistry, University of Leicester
2007-2010 Wellcome Trust Postdoc, University of Oxford
2011-2014 BBSRC Researcher Co-Investigator, University of Oxford
2015-2019 Research Fellow, University of Oxford
2019- Associate Professor (Reader) Computational Biochemistry, University of Warwick

Current projects Research interests

One of the fundamental challenges in biological sciences is to visualise biomolecular machines in high-resolution detail. This is notoriously difficult, expensive and time-consuming to achieve by using experimental techniques, especially for proteins that exist in cell membranes, known as Integral membrane proteins (IMPs). These proteins play fundamental roles in cell biology e.g. as processing enzymes, ion channels, drug receptors, and solute transporters.

My group uses computational methods to study IMP structures and currently hosts MemProtMD; a pipeline for inserting experimentally-solved IMP structures into their native bilayer environment and analysing the stability, dynamics and resultant lipid interactions. This resource uses multiscale molecular dynamics (MD) simulations that permit the accurate assembly of an IMP into a membrane at the coarse-grain level, prior to careful assessment of the quality of the IMP structure at atomic resolution.

The MemProtMD pipeline also forms a springboard to studying the dynamics of experimentally solved structures through MD simulations. With the increasing threat of anti-microbial resistance, we are especially interested in bacterial IMPs. Knowledge of the three-dimensional structures of proteins involved in essential processes provides the physical details of potentially viable targets for killing drug-resistant, pathogenic bacteria. By breathing life into these frozen structures we may assess the association of proteins with lipids, drug molecules and other components of the protein complexes.

Publications

Journals

Herdman, M., 'von Ku?gelgen, A., 'Kureisaite-Ciziene, D., 'Duman, R., 'El Omari, K., 'Garman, E. F., 'Kjaer, A., 'Kolokouris, D., 'Lo?we, J., 'Wagner, A., 'Stansfeld, Phillip J., 'Bharat, T., 2022. 'High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells. Structure, 30 (2), pp. 215-228
'Song, Wanling, 'Corey, Robin, 'Ansell, Tillson, 'Cassidy, C. Keith, 'Horrell, Michael, 'Duncan, Anna, 'Stansfeld, Phillip J., 'Sansom, Mark S. P., 2022. 'PyLipID : A Python package for analysis of protein-lipid interactions from MD simulations. Journal of Chemical Theory and Computation, 18 (2), pp. 1188-1201
'Ashraf, Khuram U., 'Nygaard, Rie, 'Vickery, Owen N., 'Erramilli, Satchal K., 'Herrera, Carmen M., 'McConville, Thomas H., 'Petrou, Vasileios I., 'Giacometti, Sabrina I., 'Belcher Dufrisne, Meagan, 'Nosol, Kamil, 'Zinkle, Allen P., 'Graham, Chris L. B., 'Loukeris, Michael, 'Kloss, Brian, 'Skorupinska-Tudek, Karolina, 'Swiezewska, Ewa, 'Roper, David I., 'Clarke, Oliver B., 'Uhlemann, Anne-Catrin, 'Kossiakof, Anthony A. et al (Select to open full list), 2022. 'Structural basis of lipopolysaccharide maturation by the WaaL O-antigen ligase. Nature, 604, pp. 371-376
'Oluwole, Abraham O., 'Corey, Robin A., 'Brown, Chelsea M., 'Hern?ndez-Rocamora, Victor M., 'Stansfeld, Phillip J., 'Vollmer, Waldemar, 'Bolla, Jani R., 'Robinson, Carol V., 2022. 'Peptidoglycan biosynthesis is driven by lipid transfer along enzyme-substrate affinity gradients. Nature Communications, 13 (1)
'Maloney, Finn P., 'Kuklewicz, Jeremi, 'Corey, Robin A., 'Bi, Yunchen, 'Ho, Ruoya, 'Mateusiak, Lukasz, 'Pardon, Els, 'Steyaert, Jan, 'Stansfeld, Phillip J., 'Zimmer, Jochen, 2022. 'Structure, substrate-recognition, and initiation of hyaluronan synthase. Nature, 604, pp. 195-201
'Caldwell, Tracy A., 'Vickery, Owen N., 'Colburn, Jonathan David, 'Stansfeld, Phillip J., 'Columbus, Linda, 2022. 'Conformational dynamics of the membrane enzyme LspA upon antibioticand substrate binding. Biophysical Journal, 121 (11), pp. 2078-2083
'Williams, Sophie I, 'Yu, Xiulian, 'Ni, Tao, 'Gilbert, Robert J C, 'Stansfeld, Phillip J., 2022. 'Structural, functional and computational studies of membrane recognition by Plasmodium Perforin-Like Proteins 1 and 2. Journal of molecular biology, 434 (13)
'Radhakrishnan, Anjana, 'Brown, Chelsea M., 'Guy, Collette S., 'Cooper, Charlotte, 'Pacheco-G?mez, Ra?l, 'Stansfeld, Phillip J., 'Fullam, Elizabeth, 2022. 'Interrogation of the pathogen box reveals small molecule ligands against the mycobacterial trehalose transporter LpqY-SugABC. RSC Medicinal Chemistry
'Renne, Mike, 'Corey, Robin, 'Ver?ssimo Ferreira, Joana, 'Stansfeld, Phillip J., 'Carvalho, Pedro, 2022. 'Seipin concentrates distinct neutral lipids via interactions with their acyl chain carboxyl esters. Journal of Cell Biology, 221 (9)
Burt, Alister, Cassidy, C. Keith, Stansfeld, Phillip J., Gutsche, Irina, 2021. Alternative architecture of the E. coli chemosensory array. Biomolecules, 11 (4)
Pipatpolkai, Tanadet, Quetschlich, Daniel, Stansfeld, Phillip J., 2021. From bench to biomolecular simulation : phospholipid modulation of potassium channels. Journal of Molecular Biology
Perkins, Arden, Tudorica, Dan A., Teixeira, Raphael D., Schirmer, Tilman, Zumwalt, Lindsay, Ogba, O. Maduka, Cassidy, C. Keith, Stansfeld, Phillip J., Guillemin, Karen, Cooper, Vaughn S., 2021. A bacterial inflammation sensor regulates c-di-GMP signaling, adhesion, and biofilm formation. mBio, 12 (3)
Vickery, Owen N., Stansfeld, Phillip J., 2021. CG2AT2 : an enhanced fragment-based approach for serial multi-scale molecular dynamics simulations. Journal of Chemical Theory and Computation, 17 (10), pp. 6472-6482
Ansell, T. Bertie, Curran, Luke, Horrell, Michael R., Pipatpolkai, Tanadet, Letham, Suzanne C., Song, Wanling, Siebold, Christian, Stansfeld, Phillip J., Sansom, Mark S. P., Corey, Robin A., 2021. Relative affinities of protein?cholesterol interactions from equilibrium molecular dynamics simulations. Journal of Chemical Theory and Computation, 17 (10), pp. 6548-6558
Klug, Yoel A., Deme, Justin C., Corey, Robin A., Renne, Mike F., Stansfeld, Phillip J., Lea, Susan M., Caralho, Pedro, 2021. Mechanism of lipid droplet formation by the yeast Sei1/Ldb16 Seipin complex. Nature Communications, 12
Furze, Christopher M., Delso, Ignacio, Casal, Enriqueta, Guy, Collette S., Seddon, Chloe, Brown, Chelsea M., Parker, Hadyn L., Radhakrishnan, Anjana, Pacheco-Gomez, Raul, Stansfeld, Phillip J., Angulo, Jesus, Cameron, Alexander D., Fullam, Elizabeth, 2021. Structural basis of trehalose recognition by the mycobacterial LpqY-SugABC transporter. Journal of Biological Chemistry, 296
Corey, Robin A., Song, Wanling, Duncan, Anna L., Ansell, T. Bertie, Sansom, Mark S. P., Stansfeld, Phillip J., 2021. Identification and assessment of cardiolipin interactions with E. coli inner membrane proteins. Science Advances, 7 (34)
Silberberg, Jakob M., Corey, Robin A., Hielkema, Lisa, Stock, Charlott, Stansfeld, Phillip J., Paulino, Cristina, Hänelt, Inga, 2021. Deciphering ion transport and ATPase coupling in the intersubunit tunnel of KdpFABC. Nature Communications, 12 (1)
Moseng, Mitchell A., Lyu, Meinan, Pipatpolkai, Tanadet, Glaza, Przemyslaw, Emerson, Corey C., Stewart, Phoebe L., Stansfeld, Phillip J., Yu, Edward W., 2021. Cryo-EM structures of CusA reveal a mechanism of metal-ion export. mBio, 12 (2)
Simcock, Patrick W., Bublitz, Maike, Cipcigan, Flaviu, Ryadnov, Maxim G., Crain, Jason, Stansfeld, Phillip J., Sansom, Mark S. P., 2021. Membrane binding of antimicrobial peptides is modulated by lipid charge modification. Journal of Chemical Theory and Computation, 17 (2), pp. 1218-1228
'Quetschlich, Daniel, 'Esser, Tim K., 'Newport, Thomas D., 'Fiorentino, Francesco, 'Shutin, Denis, 'Chen, Siyun, 'Davis, Rachel, 'Lovera, Silvia, 'Liko, Idlir, 'Stansfeld, Phillip J., 'Robinson, Carol V., 'Martelli, Pier Luigi, 2021. 'NaViA : a program for the visual analysis of complex mass spectra. Bioinformatics, 37 (24), pp. 4876-4878
'Dinsdale, Ria L., 'Pipatpolkai, Tanadet, 'Agostinelli, Emilio, 'Russell, Angela J., 'Stansfeld, Phillip J., 'Tammaro, Paolo, 2021. 'An outer-pore gate modulates the pharmacology of the TMEM16A channel. Proceedings of the National Academy of Sciences of the United States of America, 118 (34)
'Hammond, Katharine, 'Cipcigan, Flaviu, 'Al Nahas, Kareem, 'Losasso, Valeria, 'Lewis, Helen, 'Cama, Jehangir, 'Martelli, Fausto, 'Simcock, Patrick W., 'Fletcher, Marcus, 'Ravi, Jascindra, 'Stansfeld, Phillip J., 'Pagliara, Stefano, 'Hoogenboom, Bart W., 'Keyser, Ulrich F., 'Sansom, Mark S. P., 'Crain, Jason, 'Ryadnov, Maxim G., 2021. 'Switching cytolytic nanopores into antimicrobial fractal ruptures by a single side chain mutation. ACS Nano, 15 (6), pp. 9679-9689
Ni, Tao, Jiao, Fang, Yu, Xiulian, Aden, Sa?a, Ginger, Lucy, Williams, Sophie, Bai, Fangfang, Pra?ák, Vojtech, Karia, Dimple, Stansfeld, Phillip J., Zhang, Peijun, Munson, George, Anderluh, Gregor, Scheuring, Simon, Gilbert, Robert J. C., 2020. Structure and mechanism of bactericidal mammalian perforin-2, an ancient agent of innate immunity. Science Advances, 6 (5)
Burt, Alister, Cassidy, C. Keith, Ames, Peter, Bacia-Verloop, Maria, Baulard, Megghane, Huard, Karine, Luthey-Schulten, Zaida, Desfosses, Ambroise, Stansfeld, Phillip J., Margolin, William, Parkinson, John S., Gutsche, Irina, 2020. Complete structure of the chemosensory array core signalling unit in an E. coli 1 minicell strain. Nature Communications, 11
Bolla, Jani Reddy, Corey, Robin A., Sahin, Cagla, Gault, Joseph, Hummer, Alissa, Hopper, Jonathan T. S., Lane, David P., Drew, David, Allison, Timothy M., Stansfeld, Phillip J., Robinson, Carol V., Landreh, Michael, 2020. A mass spectrometry-based approach to distinguish annular and specific lipid binding to membrane proteins. Angewandte Chemie International Edition, 59 (9), pp. 3523-3528
Cassidy, Keith, Himes, Benjamin A., Sun, Dapeng, Ma, Jun, Zhao, Gongpu, Parkinson, John S., Stansfeld, Phillip J., Luthey-Schulten, Z., Zhang, Peijun, 2020. Structure and dynamics of the E. coli chemotaxis core signaling complex by cryo-electron tomography and molecular simulations. Communications Biology, 3 (24)
Corey, Robin A., Stansfeld, Phillip J., Sansom, Mark S. P., 2020. The energetics of protein-lipid interactions as viewed by molecular simulations. Biochemical Society Transactions, 48 (1), pp. 25-37
von Kügelgen, Andriko, Tang, Haiping, Hardy, Gail G., Kureisaite-Ciziene, Danguole, Brun, Yves V., Stansfeld, Phillip J., Robinson, Carol V., Bharat, Tanmay A. M., 2020. In situ structure of an intact lipopolysaccharide-bound bacterial surface layer. Cell, 180 (2), pp. P348-358.E15
Pye, Valerie E., Rosa, Annachiara, Bertelli, Cinzia, Struwe, Weston B., Maslen, Sarah L., Corey, Robin A., Liko, Idlir, Hassall, Mark, Mattiuzzo, Giada, Ballandras-Colas, Allison, Nans, Andrea, Takeuchi, Yasuhiro, Stansfeld, Phillip J., Skehel, J. Mark, Robinson, Carol V., Pizzato, Massimo, Cherepanov, Peter, 2020. A bipartite structural organization defines the SERINC family of HIV-1 restriction factors. Nature Structural & Molecular Biology, 27, pp. 78-83
Rao, Shanlin, Bates, George T., Matthews, Callum R., Newport, Thomas D., Vickery, Owen N., Stansfeld, Phillip J., 2020. Characterising membrane association and periplasmic transfer of bacterial lipoproteins through molecular dynamics simulations. Structure, 28 (4), pp. 475-487
Yamamoto, Eiji, Domanski,, Jan, Naughton, Fiona B., Best , Robert B., Kalli, Antreas C., Stansfeld, Phillip J., Sansom, Mark S. P., 2020. Multiple lipid binding sites determine the affinity of PH domains for phosphoinositide-containing membranes. Science Advances, 6 (8)
Domanski, Jan, Sansom, Mark S. P., Stansfeld, Phillip J., Best, Robert B., 2020. Atomistic mechanism of transmembrane helix association. PLOS Computational Biology, 16 (6)
Dijkman, Patricia M., Muñoz-García, Juan C., Steven R., Lavington, Suemy Kumagai, Patricia, dos Reis, Rosana I., Yin, Daniel, Stansfeld, Phillip J., Costa-Filho, Antonio José, Watt , Anthony, 2020. Conformational dynamics of a G protein-coupled receptor helix 8 in lipid membranes. Science Advances, 6 (33)
Pipatpolkai, Tanadet, Usher, Samuel, Stansfeld, Phillip J., Ashcroft, Frances M., 2020. New insights into KATP channel gene mutations and neonatal diabetes mellitus. Nature Reviews Endocrinology, 16 (7), pp. 378-393
Cook, Jonathan P., Baverstock, Tyler, McAndrew, Martin B. L., Stansfeld, Phillip J., Roper, David I., Crow, Allister, 2020. Insights into intrinsic resistance and bacterial cell division from a structure of EnvC bound to the FtsX periplasmic domain. Proceedings of the National Academy of Sciences of the United States of America, 117 (45), pp. 28355-28365
Baker, Alexander, Richards, Sarah-Jane, Guy, Collette S., Congdon, Thomas R., Hasan, Muhammad, Zwetsloot, Alexander J., Gallo, Angelo, Lewandowski, Józef R., Stansfeld, Phillip J., Straube, Anne, Walker, Marc, Chessa, Simona, Pergolizzi, Giulia, Dedola, Simone, Field, Robert A., Gibson, Matthew I., 2020. The SARS-COV-2 spike protein binds sialic acids and enables rapid detection in a lateral flow point of care diagnostic device. ACS Central Science, 6 (11), pp. 2046-2052
Palmer, Tracy, Stansfeld, Phillip J., 2020. Targeting of proteins to the twin-arginine transport pathway. Molecular Microbiology, 113 (5), pp. 861-871
Fiorentino, Francesco, Sauer, Joshua B., Qiu, XingYu, Corey, Robin A., Cassidy, Keith, Mynors-Wallis, Benjamin, Mehmood, Shahid, Bolla, Jani R., Stansfeld, Phillip J., Robinson, Carol V., 2020. Dynamics of an LPS translocon induced by substrate and an antimicrobial peptide. Nature Chemical Biology, 17, pp. 187-195
Stansfeld, Phillip J., 2020. Greasing the gears of mechanosensitive piezo channels with phosphoinositides and cholesterol. Biophysical Journal, 119 (8), pp. 1467-1469
Deme, Justin C., Johnson, Steven, Vickery, Owen N., Muellbauer, Amy, Monkhouse, Holly, Griffiths, Thomas, Hennell James, Rory, Berks, Ben C., Coulton, James W., Stansfeld, Phillip J., Lea, Susan M., 2020. Structures of the stator complex that drives rotation of the bacterial flagellum. Nature microbiology, 5, pp. 1553-1564
Tohidifar, Payman, Bodhankar,, Girija, Pei, Sichong, Cassidy, C. Keith, Walukiewicz, Hanna, Orodal, George, Stansfeld, Phillip J., Rao, Christopher, 2020. The unconventional cytoplasmic sensing mechanism for ethanol chemotaxis in Bacillus subtilis. mBio, 11
Pipatpolkai, Tanadet, Corey, Robin A., Proks, Peter, Ashcroft, Frances M., Stansfeld, Phillip J., 2020. Evaluating inositol phospholipid interactions with inward rectifier potassium channels and characterising their role in disease. Communications chemistry, 3
Tascón, Igor, Sousa, Joana S., Corey, Robin A., Mills, Deryck J., Griwatz, David, Aumüller, Nadine, Mikusevic, Vedrana, Stansfeld, Phillip J., Vonck, Janet, Hänelt, Inga, 2020. Structural basis of proton-coupled potassium transport in the KUP family. Nature Communications, 11 (1)
Rao, Shanlin, Klesse, Gianni, Stansfeld, Phillip J., Tucker, Stephen J., Sansom, Mark S. P., 2019. A heuristic derived from analysis of the ion channel structural proteome permits the rapid identification of hydrophobic gates. Proceedings of the National Academy of Sciences of the United States of America, 116 (28), pp. 13989-13995
Rinné, Susanne, Kiper, Aytug K., Vowinkel, Kirsty S., Ramírez, David, Schewe, Marcus, Bedoya, Mauricio, Aser, Diana, Gensler, Isabella, Netter, Michael F., Stansfeld, Phillip J., Baukrowitz, Thomas, Gonzalez, Wendy, Decher, Niels, 2019. The molecular basis for an allosteric inhibition of K+-flux gating in K2P channels. eLife, 8
Caffalette, Christopher A., Corey, Robin A., Sansom, Mark S. P., Stansfeld, Phillip J., Zimmer, Jochen, 2019. A lipid gating mechanism for the channel-forming O antigen ABC transporter. Nature Communications, 10 (1)
Abraham, Mark, Apostolov, Rossen, Barnoud, Jonathan, Bauer, Paul, Blau, Christian, Bonvin, Alexandre M.J.J., Chavent, Matthieu, Chodera, John, Condic-Jurkic, Karmen, Delemotte, Lucie, Grubmüller, Helmut, Howard, Rebecca J., Jordan, E. Joseph, Lindahl, Erik, Ollila, O. H. Samuli, Selent, Jana, Smith, Daniel G. A., Stansfeld, Phillip J., Tiemann, Johanna K.S., Trellet, Mikael et al (Select to open full list), 2019. Sharing data from molecular simulations. Journal of Chemical Information and Modeling, 59 (10), pp. 4093-4099
Bushell, Simon R., Pike, Ashley C. W., Falzone, Maria E., Rorsman, Nils J. G., Ta, Chau M., Corey, Robin A., Newport, Thomas D., Christianson, John C., Scofano, Lara F., Shintre, Chitra A., Tessitore, Annamaria, Chu, Amy, Wang, Qinrui, Shrestha, Leela, Mukhopadhyay, Shubhashish M. M., Love, James D., Burgess-Brown, Nicola A., Sitsapesan, Rebecca, Stansfeld, Phillip J., Huiskonen, Juha T. et al (Select to open full list), 2019. The structural basis of lipid scrambling and inactivation in the endoplasmic reticulum scramblase TMEM16K. Nature Communications, 10 (1)
Corey, Robin A., Vickery, Owen N., Sansom, Mark S. P., Stansfeld, Phillip J., 2019. Insights into membrane protein?lipid interactions from free energy calculations. Journal of Chemical Theory and Computation, 15 (10), pp. 5727-5736
Parker, Joanne L., Corey, Robin A., Stansfeld, Phillip J., Newstead, Simon, 2019. Structural basis for substrate speci?city and regulation of nucleotide sugar transporters in the lipid bilayer. Nature Communications, 10
Autzen, Henriette E., Koldsø, Heidi, Stansfeld, Phillip J., Gourdon, Pontus, Sansom, Mark S. P., Nissen, Poul, 2018. Interactions of a bacterial Cu(I)-ATPase with a complex lipid environment. Biochemistry, 57 (28), pp. 4063-4073
Chavent, Matthieu, Karia, Dimple, Kalli, Antreas C., Domanski, Jan, Duncan, Anna L., Hedger, George, Stansfeld, Phillip J., Seiradake, Elena, Jones, E. Yvonne, Sansom, Mark S. P., 2018. Interactions of the EphA2 kinase domain with PIPs in membranes : implications for receptor function. Structure, 26 (7), pp. 1025-1034.e2
Liko, Idlir, Degiacomi, Matteo T., Lee, Sejeong, Newport, Thomas D., Gault, Joseph, Reading, Eamonn, Hopper, Jonathan T. S., Housden, Nicholas G., White, Paul, Colledge, Matthew, Sula, Altin, Wallace, B. A., Kleanthous, Colin, Stansfeld, Phillip J., Bayley, Hagan, Benesch, Justin L. P., Allison, Timothy M., Robinson, Carol V., 2018. Lipid binding attenuates channel closure of the outer membrane protein OmpF. Proceedings of the National Academy of Sciences of the United States of America, 115 (26), pp. 6691-6696
Rao, Shanlin, Lynch, Charlotte I., Klesse, Gianni, Oakley, Georgia E., Stansfeld, Phillip J., Tucker, Stephen J., Sansom, Mark S. P., 2018. Water and hydrophobic gates in ion channels and nanopores. Faraday Discussions, 209, pp. 231-247
Domanski, Jan, Sansom, Mark S. P., Stansfeld, Phillip J., Best, Robert B., 2018. Balancing force field protein?lipid interactions to capture transmembrane Helix?Helix association. Journal of Chemical Theory and Computation, 14 (3), pp. 1706-1715
Newport, Thomas D., Sansom, Mark S. P., Stansfeld, Phillip J., 2018. The MemProtMD database : a resource for membrane-embedded protein structures and their lipid interactions. Nucleic Acids Research, 47 (D1), pp. D390-D397
Ni, Tao, Williams, Sophie I., Rezelj, Sa?a, Anderluh, Gregor, Harlos, Karl, Stansfeld, Phillip J., Gilbert, Robert J. C., 2018. Structures of monomeric and oligomeric forms of the Toxoplasma gondiiperforin-like protein 1. Science Advances, 4 (3)
El Ghachi, Meriem, Howe, Nicole, Huang, Chia-Ying, Olieric, Vincent, Warshamanage, Rangana, Touzé, Thierry, Weichert, Dietmar, Stansfeld, Phillip J., Wang, Meitian, Kerff, Fred, Caffrey, Martin, 2018. Crystal structure of undecaprenyl-pyrophosphate phosphatase and its role in peptidoglycan biosynthesis. Nature Communications, 9 (1)
Stansfeld, Phillip J., 2017. Computational studies of membrane proteins : from sequence to structure to simulation. Current Opinion in Structural Biology, 45, pp. 133-141
Tolia, Niraj H, Cutts, Erin E., Laasch, Niklas, Reiter, Dirk M., Trenker, Raphael, Slater, Leanne M., Stansfeld, Phillip J., Vakonakis, Ioannis, 2017. Structural analysis of P. falciparum KAHRP and PfEMP1 complexes with host erythrocyte spectrin suggests a model for cytoadherent knob protrusions. PLoS Pathogens, 13 (8)
Rao, Shanlin, Klesse, Gianni, Stansfeld, Phillip J., Tucker, Stephen J., Sansom, Mark S. P., 2017. A BEST example of channel structure annotation by molecular simulation. Channels, 11 (4), pp. 347-353
Domanski, Jan, Hedger, George, Best, Robert B., Stansfeld, Phillip J., Sansom, Mark S. P., 2017. Convergence and sampling in determining free energy landscapes for membrane protein association. The Journal of Physical Chemistry B, 121 (15), pp. 3364-3375
Habersetzer, Johann, Moore, Kristoffer, Cherry, Jon, Buchanan, Grant, Stansfeld, Phillip J., Palmer, Tracy, 2017. Substrate-triggered position switching of TatA and TatB during Tat transport in Escherichia coli. Open Biology, 7 (8)
Wiktor, Maciej, Weichert, Dietmar, Howe, Nicole, Huang, Chia-Ying, Olieric, Vincent, Boland, Coilín, Bailey, Jonathan, Vogeley, Lutz, Stansfeld, Phillip J., Buddelmeijer, Nienke, Wang, Meitian, Caffrey, Martin, 2017. Structural insights into the mechanism of the membrane integral N-acyltransferase step in bacterial lipoprotein synthesis. Nature Communications, 8 (1)
Gupta, Kallol, Donlan, Joseph A. C., Hopper, Jonathan T. S., Uzdavinys, Povilas, Landreh, Michael, Struwe, Weston B., Drew, David, Baldwin, Andrew J., Stansfeld, Phillip J., Robinson, Carol V., 2017. The role of interfacial lipids in stabilizing membrane protein oligomers. Nature, 541 (7637), pp. 421-424
Alcock, Felicity, Stansfeld, Phillip J., Basit, Hajra, Habersetzer, Johann, Baker, Matthew A. B., Palmer, Tracy, Wallace, Mark I., Berks, Ben C., 2016. Assembling the tat protein translocase. eLife, 5
Lörinczi, Eva, Helliwell, Matthew, Finch, Alina, Stansfeld, Phillip J., Davies, Noel W., Mahaut-Smith, Martyn, Muskett, Frederick W., Mitcheson, John S., 2016. Calmodulin regulates human ether à Go-Go 1 (hEAG1) potassium channels through interactions of the eag domain with the cyclic nucleotide binding homology domain. Journal of Biological Chemistry, 291 (34), pp. 17907-17918
Bolivar, Juan H., Muñoz-García, Juan C., Castro-Dopico, Tomas, Dijkman, Patricia M., Stansfeld, Phillip J., Watts, Anthony, 2016. Interaction of lipids with the neurotensin receptor 1. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1858 (6), pp. 1278-1287
Vogeley, L., El Arnaout, T., Bailey, J., Stansfeld, Phillip J., Boland, C., Caffrey, M., 2016. Structural basis of lipoprotein signal peptidase II action and inhibition by the antibiotic globomycin. Science, 351 (6275), pp. 876-880
Gu, Yinghong, Li, Huanyu, Dong, Haohao, Zeng, Yi, Zhang, Zhengyu, Paterson, Neil G., Stansfeld, Phillip J., Wang, Zhongshan, Zhang, Yizheng, Wang, Wenjian, Dong, Changjiang, 2016. Structural basis of outer membrane protein insertion by the BAM complex. Nature, 531 (7592), pp. 64-69
Paci, Emanuele, Sharma, Satyan, Kim, Brian N., Stansfeld, Phillip J., Sansom, Mark S. P., Lindau, Manfred, 2015. A coarse grained model for a lipid membrane with physiological composition and leaflet asymmetry. PLoS One, 10 (12)
Wojdyla, Justyna A., Cutts, Erin, Kaminska, Renata, Papadakos, Grigorios, Hopper, Jonathan T. S., Stansfeld, Phillip J., Staunton, David, Robinson, Carol V., Kleanthous, Colin, 2015. Structure and function of the escherichia coli Tol-Pal stator protein TolR. Journal of Biological Chemistry, 290 (44), pp. 26675-26687
Cutts, E. E., Inglis, A., Stansfeld, Phillip J., Vakonakis, I., Hatzopoulos, G. N., 2015. The centriolar protein CPAP G-box : an amyloid fibril in a single domain. Biochemical Society Transactions, 43 (5), pp. 838-843
Cléon, François, Habersetzer, Johann, Alcock, Felicity, Kneuper, Holger, Stansfeld, Phillip J., Basit, Hajra, Wallace, Mark I., Berks, Ben C., Palmer, Tracy, 2015. The TatC component of the twin-arginine protein translocase functions as an obligate oligomer. Molecular Microbiology, 98 (1), pp. 111-129
Stansfeld, Phillip J., Goose, Joseph E., Caffrey, Martin, Carpenter, Elisabeth P., Parker, Joanne L., Newstead, Simon, Sansom, Mark S. P., 2015. MemProtMD : automated insertion of membrane protein structures into explicit lipid membranes. Structure, 23 (7), pp. 1350-1361
Gu, Yinghong, Stansfeld, Phillip J., Zeng, Yi, Dong, Haohao, Wang, Wenjian, Dong, Changjiang, 2015. Lipopolysaccharide is inserted into the outer membrane through an intramembrane hole, a lumen gate, and the lateral opening of LptD. Structure, 23 (3), pp. 496-504
Li, Dianfan, Stansfeld, Phillip J., Sansom, Mark S. P., Keogh, Aaron, Vogeley, Lutz, Howe, Nicole, Lyons, Joseph A., Aragao, David, Fromme, Petra, Fromme, Raimund, Basu, Shibom, Grotjohann, Ingo, Kupitz, Christopher, Rendek, Kimberley, Weierstall, Uwe, Zatsepin, Nadia A., Cherezov, Vadim, Liu, Wei, Bandaru, Sateesh, English, Niall J. et al (Select to open full list), 2015. Ternary structure reveals mechanism of a membrane diacylglycerol kinase. Nature Communications, 6 (1)
Berks, Ben C., Lea, Susan M., Stansfeld, Phillip J., 2014. Structural biology of Tat protein transport. Current Opinion in Structural Biology, 27, pp. 32-37
Chavent, Matthieu, Chetwynd, Alan P., Stansfeld, Phillip J., Sansom, Mark S. P., 2014. Dimerization of the EphA1 receptor tyrosine kinase transmembrane domain : insights into the mechanism of receptor activation. Biochemistry, 53 (42), pp. 6641-6652
Dong, Haohao, Xiang, Quanju, Gu, Yinghong, Wang, Zhongshan, Paterson, Neil G., Stansfeld, Phillip J., He, Chuan, Zhang, Yizheng, Wang, Wenjian, Dong, Changjiang, 2014. Structural basis for outer membrane lipopolysaccharide insertion. Nature, 511 (7507), pp. 52-56
Rothwell, Simon W., Stansfeld, Phillip J., Bragg, Laricia, Verkhratsky, Alexej, North, R. Alan, 2013. Direct gating of ATP-activated ion channels (P2X2 receptors) by lipophilic attachment at the outer end of the second transmembrane domain. Journal of Biological Chemistry, 289 (2), pp. 618-626
Hatzopoulos, Georgios N., Erat, Michèle C., Cutts, Erin, Rogala, Kacper B., Slater, Leanne M., Stansfeld, Phillip J., Vakonakis, Ioannis, 2013. Structural analysis of the G-Box domain of the microcephaly protein CPAP suggests a role in centriole architecture. Structure, 21 (11), pp. 2069-2077
Marzian, Stefanie, Stansfeld, Phillip J., Rapedius, Markus, Rinné, Susanne, Nematian-Ardestani, Ehsan, Abbruzzese, Jennifer L., Steinmeyer, Klaus, Sansom, Mark S. P., Sanguinetti, Michael C., Baukrowitz, Thomas, Decher, Niels, 2013. Side pockets provide the basis for a new mechanism of Kv channel?specific inhibition. Nature Chemical Biology, 9 (8), pp. 507-513
Shintre, C. A., Pike, A. C. W., Li, Q., Kim, J-I., Barr, A. J., Goubin, S., Shrestha, L., Yang, J., Berridge, G., Ross, J., Stansfeld, Phillip J., Sansom, M. S. P., Edwards, A. M., Bountra, C., Marsden, B. D., von Delft, F., Bullock, A. N., Gileadi, O., Burgess-Brown, N. A., Carpenter, E. P., 2013. Structures of ABCB10, a human ATP-binding cassette transporter in apo- and nucleotide-bound states. Proceedings of the National Academy of Sciences of the United States of America, 110 (24), pp. 9710-9715
Stansfeld, Phillip J., Jefferys, Elizabeth E., Sansom, Mark S. P., 2013. Multiscale simulations reveal conserved patterns of lipid interactions with aquaporins. Structure, 21 (5), pp. 810-819
Quigley, A., Dong, Y. Y., Pike, A. C. W., Dong, L., Shrestha, L., Berridge, G., Stansfeld, Phillip J., Sansom, M. S. P., Edwards, A. M., Bountra, C., von Delft, F., Bullock, A. N., Burgess-Brown, N. A., Carpenter, E. P., 2013. The structural basis of ZMPSTE24-dependent laminopathies. Science, 339 (6127), pp. 1604-1607
Schmidt, Matthias R., Stansfeld, Phillip J., Tucker, Stephen J., Sansom, Mark S. P., 2013. Simulation-based prediction of phosphatidylinositol 4,5-Bisphosphate binding to an ion channel. Biochemistry, 52 (2), pp. 279-281
Rollauer, Sarah E., Tarry, Michael J., Graham, James E., Jääskeläinen, Mari, Jäger, Franziska, Johnson, Steven, Krehenbrink, Martin, Liu, Sai-Man, Lukey, Michael J., Marcoux, Julien, McDowell, Melanie A., Rodriguez, Fernanda, Roversi, Pietro, Stansfeld, Phillip J., Robinson, Carol V., Sansom, Mark S. P., Palmer, Tracy, Högbom, Martin, Berks, Ben C., Lea, Susan M., 2012. Structure of the TatC core of the twin-arginine protein transport system. Nature, 492 (7428), pp. 210-214
Rapedius, Markus, Schmidt, Matthias R., Sharma, Chetan, Stansfeld, Phillip J., Sansom, Mark S. P., Baukrowitz, Thomas, Tucker, Stephen J., 2012. State-independent intracellular access of quaternary ammonium blockers to the pore of TREK-1. Channels, 6 (6), pp. 473-478
Wemhöner, Konstantin, Silbernagel, Nicole, Marzian, Stefanie, Netter, Michael F., Rinné, Susanne, Stansfeld, Phillip J., Decher, Niels, 2012. A leucine zipper motif essential for gating of hyperpolarization-activated channels. Journal of Biological Chemistry, 287 (48), pp. 40150-40160
Andres-Enguix, Isabelle, Shang, Lijun, Stansfeld, Phillip J., Morahan, Julia M., Sansom, Mark S. P., Lafrenière, Ronald G., Roy, Bishakha, Griffiths, Lyn R., Rouleau, Guy A., Ebers, George C., Cader, Zameel M., Tucker, Stephen J., 2012. Functional analysis of missense variants in the TRESK (KCNK18) K+ channel. Scientific Reports, 2 (1)
Stansfeld, Phillip J., Sansom, Mark S.P., 2011. Molecular simulation approaches to membrane proteins. Structure, 19 (11), pp. 1562-1572
Lumb, Craig N., He, Ju, Xue, Yi, Stansfeld, Phillip J., Stahelin, Robert V., Kutateladze, Tatiana G., Sansom, Mark S.P., 2011. Biophysical and computational studies of membrane penetration by the GRP1 Pleckstrin Homology Domain. Structure, 19 (9), pp. 1338-1346
Piechotta, Paula L., Rapedius, Markus, Stansfeld, Phillip J., Bollepalli, Murali K., Erhlich, Gunter, Andres-Enguix, Isabelle, Fritzenschaft, Hariolf, Decher, Niels, Sansom, Mark S. P., Tucker, Stephen J., Baukrowitz, Thomas, 2011. The pore structure and gating mechanism of K2P channels. The EMBO Journal, 30 (17), pp. 3607-3619
Männikkö, Roope, Stansfeld, Phillip J., Ashcroft, Alexandra S., Hattersley, Andrew T., Sansom, Mark S. P., Ellard, Sian, Ashcroft, Frances M., 2011. A conserved tryptophan at the membrane-water interface acts as a gatekeeper for Kir6.2/SUR1 channels and causes neonatal diabetes when mutated. The Journal of Physiology, 589 (13), pp. 3071-3083
Streit, Anne K., Netter, Michael F., Kempf, Franca, Walecki, Magdalena, Rinné, Susanne, Bollepalli, Murali K., Preisig-Müller, Regina, Renigunta, Vijay, Daut, Jürgen, Baukrowitz, Thomas, Sansom, Mark S. P., Stansfeld, Phillip J., Decher, Niels, 2011. A specific two-pore domain potassium channel blocker defines the structure of the TASK-1 Open Pore. Journal of Biological Chemistry, 286 (16), pp. 13977-13984
Stansfeld, Phillip J., Sansom, Mark S. P., 2011. From coarse grained to atomistic : a serial multiscale approach to membrane protein simulations. Journal of Chemical Theory and Computation, 7 (4), pp. 1157-1166
Muskett, Frederick W., Thouta, Samrat, Thomson, Steven J., Bowen, Alexander, Stansfeld, Phillip J., Mitcheson, John S., 2010. Mechanistic insight into Humanether-à-go-go-related gene (hERG) K+Channel deactivation gating from the solution structure of the EAG domain. Journal of Biological Chemistry, 286 (8), pp. 6184-6191
Domanski, Jan, Stansfeld, Phillip J., Sansom, Mark S. P., Beckstein, Oliver, 2010. Lipidbook : a public repository for force-field parameters used in membrane simulations. Journal of Membrane Biology, 236 (3), pp. 255-258
Stansfeld, Phillip J., Hopkinson, Richard, Ashcroft, Frances M., Sansom, Mark S. P., 2009. PIP2-Binding site in Kir channels : definition by multiscale biomolecular simulations. Biochemistry, 48 (46), pp. 10926-10933
Rouse, Sarah L., Carpenter, Timothy, Stansfeld, Phillip J., Sansom, Mark S. P., 2009. Simulations of the BM2 proton channel transmembrane domain from influenza virus B. Biochemistry, 48 (42), pp. 9949-9951
Tai, Kaihsu, Stansfeld, Phillip J., Sansom, Mark S. P., 2009. Ion-blocking sites of the Kir2.1 channel revealed by multiscale modeling. Biochemistry, 48 (36), pp. 8758-8763
Ashmole, I., Vavoulis, D. V., Stansfeld, Phillip J., Mehta, Puja R., Feng, J. F., Sutcliffe, M. J., Stanfield, P. R., 2009. The response of the tandem pore potassium channel TASK-3 (K2P9.1) to voltage : gating at the cytoplasmic mouth. The Journal of Physiology, 587 (20), pp. 4769-4783
Stansfeld, Phillip J., Grottesi, Alessandro, Sands, Zara A., Sansom, Mark S. P., Gedeck, Peter, Gosling, Martin, Cox, Brian, Stanfield, Peter R., Mitcheson, John S., Sutcliffe, Michael J., 2008. Insight into the mechanism of inactivation and pH sensitivity in potassium channels from molecular dynamics simulations. Biochemistry, 47 (28), pp. 7414-7422
Hardman, Rachael M., Stansfeld, Phillip J., Dalibalta, Sarah, Sutcliffe, Michael J., Mitcheson, John S., 2007. Activation gating of hERG potassium channels. Journal of Biological Chemistry, 282 (44), pp. 31972-31981
Yuill, K. H., Stansfeld, Phillip J., Ashmole, I., Sutcliffe, M. J., Stanfield, P. R., 2007. The selectivity, voltage-dependence and acid sensitivity of the tandem pore potassium channel TASK-1: contributions of the pore domains. Pflügers Archiv - European Journal of Physiology, 455 (2), pp. 333-348
Stansfeld, Phillip J., Gedeck, Peter, Gosling, Martin, Cox, Brian, Mitcheson, John S., Sutcliffe, Michael J., 2007. Drug block of the hERG potassium channel : insight from modeling. Proteins: Structure, Function, and Bioinformatics, 68 (2), pp. 568-580
Stansfeld, Phillip J., Sutcliffe, Michael J., Mitcheson, John S., 2006. Molecular mechanisms for drug interactions with hERG that cause long QT syndrome. Expert Opinion on Drug Metabolism & Toxicology, 2 (1), pp. 81-94
Perry, Matthew, Stansfeld, Phillip J., Leaney, Joanne, Wood, Claire, de Groot, Marcel J., Leishman, Derek, Sutcliffe, Michael J., Mitcheson, John S., 2005. Drug binding interactions in the inner cavity of hERG channels : molecular insights from structure-activity relationships of clofilium and ibutilide analogs. Molecular Pharmacology, 69 (2), pp. 509-519
Chen, Philip E., Geballe, Matthew T., Stansfeld, Phillip J., Johnston, Alexander R., Yuan, Hongjie, Jacob, Amanda L., Snyder, James P., Traynelis, Stephen F., Wyllie, David J. A., 2005. Structural features of the glutamate binding site in recombinant NR1/NR2A N-Methyl-d-aspartate receptors determined by site-directed mutagenesis and molecular modeling. Molecular Pharmacology, 67 (5), pp. 1470-1484

Teaching and supervision

Phillip is the module lead for and teaches on the CH3F1 undergraduate Chemistry module

He also teaches on BS244 and BS348 in Life Sciences, and postgraduate PX923 and MIBTP masterclasses.

I am supervising the following PhD students:

Martin McAndrew (MRC DTP)
Chelsea Brown (MRC DTP)
Becca Bailey (MRC DTP)
Matyas Parrag (HetSys)
Patrick Simcock (Oxford/IBM)
Gaby Dominguez (MBio)
Ron Cvek (MChem)

Please get in touch if you would like to join the team.