Methods reporting
| Microscope (Make, Model) | Andor, Revolution |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Nikon, 100x, 1.45, oil, Nikon, 60x, 1.40, oil, Nikon, 20x, 0.75, air, |
| Light source(s) |
Select the one/s used: 405 nm, 488 nm, 561 nm, 640 nm lasers |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Dichroic: 405/488/561/640 nm or 405/488/591 nm Emission filters: DAPI - 452/45 GFP - 525/50 TRITC - 607/36 mCherry LP - 568 LP Cy5 - 685/40 GFP/mCherry high speed - 440/521/607/700 Information on other filters are available on request. |
| fpbase.org channel set-up | https://www.fpbase.org/microscope/pKZYbVWSpwqu76Jv4aEAU6/ |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Nikon Ti-Eclipse Yokogawa CSU X-10 spinning disk unit Okolabs microscope incubator Prior motorised xy stage Prior 400 um z-piezo |
| Acquisition software and settings | Andor iQ |
| Camera manufacturer, model | Andor iXon 897 |