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API Deltavision Elite DV1

DV1 is a widefield deconvolution microscope from Applied Precision Instruments (API). DV1 is optimised for live-cell, multi-colour fluorescence experiments and, combined with the in-built autofocus, is particularly suited to overnight timelapse imaging of cells and sub-cellular components. The image acquisition software (softWoRx) has deconvolution functionality which can be deployed increase contrast and resolution and improve optical sectioning.

Performance


Components

Objectives 100x oil, 60x oil, 40x oil, 20x air
LEDs 405 nm, 488 nm, 561nm, 640 nm
FRAP 410 nm, 532 nm
Cameras Photometrics CoolSNAP HQ interline CCD
Stage motorised xyz
Incubation 37 degrees, CO2
Filters DAPI, CFP, FITC, GFP, YFP, TRITC, mCherry, Cy5
Autofocus Hardware-based autofocus











Performance

PSF measurements Sept 2018

Figure 1. Optical performance monitoring by measuring the width of sub-resolution fluorescent beads (not deconvolved). The Rayleigh limit is defined here as the distance between the central peak and the first minimum. Measured for n = 5 beads in x (red), y (blue) and z (green) axes.

Excitation measurements from Sept 2018

Figure 2. Monthly measurement of the fluorescence LED illumination power output at the microscope back aperture. "Wavelength (nm)" is the wavelength at which power is measured.