DV1 methods reporting
| Microscope (Make, Model) | Applied Precision, DeltaVision Elite |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Olympus, 100x, 1.40, oil, UPlanSApo Olympus, 60x, 1.40, oil, PlanApo N Olympus, 40x, 1.40, oil, UPlanXApo (Jan 2023) old: Olympus, 40x, 1.30, oil, UPlanFL N Olympus, 20x, 0.75, air, UPlanSApo |
| Light source(s) |
Insight SSI 7 colour light engine - old. This has now (late 2022?) been replaced with a Spectra X Light Engine. Excitation wavelengths, select the one/s used: DAPI: 390/18 CFP: 438/24 GFP/FITC: 475/28 YFP: 510/10 TRITC: 543/27 mCherry: 576/25 CY-5: 632/11 |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Polychroics: Quad: Reflection 381-401:464-492:531-556:619-644 Transmission 409-456:500-523:564-611:652-700 Quad-mCh: Reflection 381-401:464-492:561-590:625-644 Transmission 409-456:500-553:598-617:652-700 C/YFP/mCh: Reflection 400-454:496-528:558-593 Transmission 463-487:537-550:602-648 Filter sets: DAPI: ex 390/18 em 435/48 CFP: ex 438/24 em 475/24 FITC/GFP: ex 475/28 em 525/48 YFP: ex 514/17 em 548/22 TRITC: ex 575/25 em 597/45 mCherry: ex 575/25 em 625/45 CY-5: ex 632/22 em 679/34 More filter information available on request. |
| fpbase.org channel set-up | not yet available |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Olympus IX-71 Precision Control microscope incubator Tokai Hit stage top incubator Applied Precision motorised xyz stage Quantitative Laser Module for FRAP Applied Precision Ultimate Focus autofocus module |
| Acquisition software and settings | softWoRx v5.5.0 |
| Camera manufacturer, model | Photometrics CoolSNAP HQ interline CCD |