Figure 1. Optical performance monitoring by measuring the width of sub-resolution fluorescent beads. The Rayleigh limit is defined here as the distance between the central peak and the first minimum. Measured for n = 5 beads in x (red), y (blue) and z (green) axes.
Figure 2. Monthly measurement of the fluorescence LED illumination power output at the microscope back aperture. "Wavelength (nm)" is the wavelength at which power is measured.