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Lattice LightSheet Microscope

The Lattice LightSheet Microscope (LLSM) has "comparable" resolution to a confocal microscope with two significant advantages: faster imaging and considerably less photo-bleaching/photo-damage. Originally developed by Nobel Laureate Dr Eric Betzig and now commercially available from 3i. Our microscope was originally Wellcome funded and is based in the Centre for Mechanochemical Cell Biology.

The microscope rapidly sweeps a thin sheet of light through the sample giving unprecedented detail of highly dynamic processes with hundreds of images a second (e.g. a 100-plane, 2-colour Z-stack in ~1 second).

The gentle imaging allows continuous recording over long time scales (hundreds to thousands of volumes).

  • Resolution with deconvolution up to 230 x 230 x 370 nm (x x y x z) [1] with 62.5x magnification
  • Imaging depth 10-50 ┬Ám depending on sample and required quality
  • Two cameras allowing imaging in multiple channels

Please acknowledge the below in your publications and presentations, in addition to the usual CAMDU acknowledgment policy.

Wellcome Trust Multi-User Equipment Grant

Award Holder: Andrew McAinsh

Award Title: Probing intra-cellular dynamics with lattice light sheet microscopy

Reference: 208384/Z/17/Z

Objectives 0.71NA LWD WI (excitation), 1.1NA WI (imaging)
Lasers 488 nm, 561nm, 642 nm
Cameras 2 x Hamamatsu ORCA-Flash 4.0 v3 sCMOS
Incubation flexible between 22 and 37 degrees Celsius, no CO2
Filters 561LP or 594LP dichroic with 605/64 and 510/42 emission filters
Sample needs

5 mm diameter circular coverslips

Please read this overviewLink opens in a new window of what to expect from a similar LLS system prior to considering use. Image processing is supported by CAMDU.

[1] Chen, B.-C. et. al. (2014). Lattice Light Sheet Microscopy: Imaging Molecules to Embryos at High Spatiotemporal Resolution. Science (New York, N.Y.), 346(6208), 1257998.

 

Visitors and Collaborators

Please see our Visitor Programme page.


If you are unsure about whether your experiment (or sample) is suitable for LLSM, feel free to send a general enquiry to .

Click here for a comparison of our microscopes.