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UltraVIEW methods reporting

Microscope (Make, Model) PerkinElmer, UltraVIEW VoX
Inverted or upright Inverted
Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any)

Select the one/s used:

Nikon, 100x, 1.40, oil, Plan Apo VC

Nikon, 60x, 1.40, oil, Plan Apo VC

Nikon, 40x, 1.30, oil, Plan Fluor

Nikon, 20x, 0.75, air, Plan Apo VC

Light source(s)

Select the one/s used:

405 nm, 488 nm, 561 nm, 640 nm lasers

Filters, dichroic mirrors, beam splitters

Select the one/s used:

Dichroic: 405/488/561/640 nm

Secondary dichroic (when using dual-camera): 580 nm longpass

Emission filters, wheel 1 (primary camera):

488 nm for imaging - 525/50

405 and 561 nm for imaging - 425/60, 615/70

640 nm for imaging - 485/60, 705/90

Emission filters, wheel 2 (secondary camera):

590 LP

Information on other filters are available on request. channel set-up
Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective

Yokogawa CSU X-10 spinning disk unit

Solent Scientific microscope incubator

Prior motorised xy stage

Prior 400 um z-piezo

Acquisition software and settings Volocity v6.3.1
Camera manufacturer, model 2 x Hamamatsu Orca-R2 interline CCD