The Olympus MPE is a multiuser multiphoton microscope funded by a BBSRC ALERT grant.
Multiphoton microscopy allows allows imaging at much greater depth than standard single-photon fluorescence imaging. It is the gold-standard for imaging in thick tissues. The Olympus MPE comes with two broad-spectrum femtosecond pulsed lasers and a resonant scanner, making it ideal for imaging multiple fluorophores at high speed. It also has an additional scanner for simultaneous photomanipulation of the smaple while imaging.
The system comes with fluorescence lifetime imaging (FLIM) capabilities from PicoQuant for quantitative biosensing experiments.
The MPE is fully motorised and has an incubator, making it excellent for imaging live samples, in particular embryos and tissues.
Located in the basement of IBRB in room IBRB03.
Hardware details for methods reporting in publications.
Absorption spectra of some dyes (no promises on the reliability of these papers):
See fpbase.org for other dyes and fluorescent proteins.
|Objectives||60x silicone, 60x oil, 30x silicone, 20x air, 10x air|
|Lasers||2 x fs pulsed lasers tunable from 690-1300 nm|
|FRAP||All available wavelengths|
|Detectors||2x PMTs, 2x GaAsPs|
|Incubation||Up to 37 degrees and CO2 (contact CAMDU if you want to use CO2)|
|Filters||Options available for GFP/RFP, CY5, SHG, NADH|
The Olympus MPE is based in the Interdisciplinary Biomedical Research Building.
If you are interested in using this system please fill out the expression of interest form or contact email@example.com for further information.