Job Title
Professor
Department
WMS - Biomedical Sciences
Phone
02476151931
Email
Web Link
Research Interests
The Royle lab researches cell biology at the molecular level. We are interested in two processes fundamental to eukaryotic cellular life: mitosis and endocytosis.
Biography
Steve is a Professor of Cell Biology at Warwick Medical School, UK. He completed his PhD in the Department of Pharmacology, University of Cambridge and moved to Leon Lagnado's lab at the MRC Laboratory of Molecular Biology, Cambridge for post-doctoral work. He started his lab as a lecturer in 2006 at the Physiological Laboratory, University of Liverpool and moved to University of Warwick in 2013.
- Ferrandiz, Nuria, Royle, Stephen J., 2023. 3D ultrastructural visualization of mitosis fidelity in human cells using serial block face scanning electron microscopy (SBF-SEM). Bio-protocol, 13 (13)
- Sittewelle, Méghane, Ferrandiz, Nuria, Fesenko, Mary, Royle, Stephen J., 2023. Genetically encoded imaging tools for investigating cell dynamics at a glance. Journal of Cell Science, 136 (7)
- 'Kuey, Cansu, 'Sittewelle, M?ghane, 'Larocque, Gabrielle, 'Hernandez-Gonz?lez, Miguel, 'Royle, Stephen J., 2022. 'Recruitment of clathrin to intracellular membranes is sufficient for vesicle formation. eLife, 11
- 'Ferrandiz, Nuria, 'Downie, Laura, 'Starling, Georgina P., 'Royle, Stephen J., 2022. 'Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes. Journal of Cell Biology, 221 (6)
- 'Larocque, Gabrielle, 'Royle, Stephen J., 2022. 'Integrating intracellular nanovesicles into integrin trafficking pathways and beyond. Cellular and Molecular Life Sciences, 79 (6)
- Smith, Sarah Marie, Larocque, Gabrielle, Wood, Katherine M., Morris, Kyle L., Roseman, Alan M., Sessions, Richard B., Royle, Stephen J., Smith, Corinne J., 2021. Multi-modal adaptor-clathrin contacts drive coated vesicle assembly. The EMBO Journal, 40 (19)
- Larocque, Gabrielle, Moore, Daniel J., Sittewelle, Méghane, Kuey, Cansu, Hetmanski, Joseph H. R., La-Borde, Penelope J., Wilson, Beverley J., Clarke, Nicholas I., Caswell, Patrick T., Royle, Stephen J., 2021. Intracellular nanovesicles mediate ?5?1 integrin trafficking during cell migration. The Journal of Cell Biology, 220 (10)
- Shah, Poonam, Chaumet, Alexandre, Royle, Stephen J., Bard, Frederic A., 2019. The NAE Pathway : autobahn to the nucleus for cell surface receptors. Cells, 8 (8)
- Küey, Cansu, Larocque, Gabrielle, Clarke, Nicholas I., Royle, Stephen J., 2019. Unintended perturbation of protein function using GFP nanobodies in human cells. Journal of Cell Science, 132 (21)
- Larocque, Gabrielle, La-Borde, Penelope J., Clarke, Nicholas I., Carter, Nicholas J., Royle, Stephen J., 2019. Tumor protein D54 defines a new class of intracellular transport vesicles. Journal of Cell Biology, 219 (1)
- Burgess, Selena G., Mukherjee, Manjeet, Sabir, Sarah, Joseph, Nimesh, Gutiérrez-Caballero, Christina, Richards, Mark W., Huguenin-Dezot, Nicolas, Chin, Jason W., Kennedy, Eileen J., Pfuhl, Mark, Royle, Stephen J., Gergely, Fanni, Bayliss, Richard, 2018. Mitotic spindle association of TACC3 requires Aurora-A-dependent stabilization of a cryptic a-helix. The EMBO Journal, 37 (8)
- Wood, Laura A., Royle, Stephen J., 2018. Imaging ?Hot-Wired? Clathrin-Mediated Endocytosis. Methods in Molecular Biology, 1847, pp. 83-94
- Clarke, Nicholas I., Royle, Stephen J., 2018. FerriTag is a new genetically-encoded inducible tag for correlative light-electron microscopy. Nature Communications, 9 (1)
- Clarke, Nicholas I., Royle, Stephen J., 2018. Correlating light microscopy with serial block face scanning electron microscopy to study mitotic spindle architecture. Methods in Cell Biology, 145, pp. 29-43
- Sarkar, Sourav, Ryan, Ellis L., Royle, Stephen J., 2017. FGFR3?TACC3 cancer gene fusions cause mitotic defects by removal of endogenous TACC3 from the mitotic spindle. Open Biology, 7 (8)
- Auckland, Philip, Clarke, Nicholas I., Royle, Stephen J., McAinsh, Andrew D., 2017. Congressing kinetochores progressively load Ska complexes to prevent force-dependent detachment. The Journal of Cell Biology, 216 (6), pp. 1623-1639
- Wood, Laura A., Larocque, Gabrielle, Clarke, Nicholas I., Sarkar, Sourav, Royle, Stephen J., 2017. New tools for ?hot-wiring? clathrin-mediated endocytosis with temporal and spatial precision. The Journal of Cell Biology, 216 (12)
- Royle, Stephen J., 2015. Super-duper resolution imaging of mitotic microtubules. Nature Reviews Molecular Cell Biology, 16 (2), pp. 67-67
- Nixon, Faye M., Gutierrez-Caballero, Cristina, Hood, Fiona E., Booth, Daniel G., Prior, Ian A., Royle, Stephen J., 2015. The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. eLife, 4, pp. 1-21
- Gutierrez-Caballero, Cristina, Burgess, Selena G., Bayliss, Richard, Royle, Stephen J., 2015. TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Biology Open, 4, pp. 170-179
- O'Regan, L., Sampson, J., Richards, M. W., Knebel, A., Roth, Daniel, Hood, F. E., Straube, Anne, Royle, Stephen J., Bayliss, R., Fry, A. M., 2015. Hsp72 is targeted to the mitotic spindle by Nek6 to promote K-fiber assembly and mitotic progression. The Journal of Cell Biology, 209 (3), pp. 349-358
- Wood, Laura A., Royle, Stephen J., 2015. Zero tolerance : amphipathic helices in endocytosis. Developmental Cell, 33 (2), pp. 119-120
- Nahorski, Michael S., Al-Gazali, Lihadh, Hertecant, Jozef, Owen, David J., Borner, Georg H. H., Chen, Ya-Chun, Benn, Caroline L., Carvalho, Ofélia P., Shaikh, Samiha S., Phelan, Anne, Robinson, Margaret S., Royle, Stephen J., Woods, C. Geoffrey, 2015. A novel disorder reveals clathrin heavy chain-22 is essential for human pain and touch development. Brain, 138 (8), pp. 2147-2160
- Willox, Anna K., Sahraoui, Yasmina M. E., Royle, Stephen J., 2014. Non-specificity of Pitstop 2 in clathrin-mediated endocytosis. Biology Open, 3 (5), pp. 326-331
- Lopez-Murcia, F. J., Royle, Stephen J., Llobet, A., 2014. Presynaptic clathrin levels are a limiting factor for synaptic transmission. Journal of Neuroscience, 34 (25), pp. 8618-8629
- Kaur, Satdip, Fielding, Andrew B., Gassner, Gisela, Carter, Nicholas J., Royle, Stephen J., 2014. An unmet actin requirement explains the mitotic inhibition of clathrin-mediated endocytosis. eLife, 3
- Royle, Stephen J., 2013. Protein adaptation : mitotic functions for membrane trafficking proteins. Nature Reviews Molecular Cell Biology, 14 (9), pp. 592-599
- Hood, Fiona E., Williams, Samantha J., Burgess, Selena G., Richards, Mark W., Roth, Daniel, Straube, Anne, Pfuhl, Mark, Bayliss, Richard, Royle, Stephen J., 2013. Coordination of adjacent domains mediates TACC3-ch-TOG-clathrin assembly and mitotic spindle binding. Journal of Cell Biology, 202 (3), pp. 463-478
- Booth, Daniel G., Cheeseman, Liam P., Prior, Ian A., Royle, Stephen J., 2013. Studying kinetochore-fiber ultrastructure using correlative light-electron microscopy. Methods in Cell Biology, 115, pp. 327-342
- Cheeseman, L. P., Harry, Edward, McAinsh, Andrew D., Prior, I. A., Royle, Stephen J., 2013. Specific removal of TACC3-ch-TOG-clathrin at metaphase deregulates kinetochore fiber tension. Journal of Cell Science, 126 (9), pp. 2102-2113
- Fielding, Andrew B., Royle, Stephen J., 2013. Mitotic inhibition of clathrin-mediated endocytosis. Cellular and Molecular Life Sciences, 70 (18), pp. 3423-3433
- Willox, Anna K., Royle, Stephen J., 2012. Functional analysis of interaction sites on the n-terminal domain of clathrin heavy chain. Traffic, 13 (1), pp. 70-81
- Willox, Anna K., Royle, Stephen J., 2012. Stonin 2 Is a major adaptor protein for clathrin-mediated synaptic vesicle retrieval. Current Biology, 22 (15), pp. 1435-1439
- Fielding, Andrew B., Willox, Anna K., Okeke, E., Royle, Stephen J., 2012. Clathrin-mediated endocytosis is inhibited during mitosis. Proceedings of the National Academy of Sciences of the United States of America, 109 (17), pp. 6572-6577
- Cheeseman, Liam P., Booth, Daniel G., Hood, Fiona E., Prior, Ian A., Royle, Stephen J., 2011. Aurora A kinase activity is required for localization of TACC3/ch-TOG/clathrin inter-microtubule bridges. Communicative & Integrative Biology, 4 (4), pp. 409-412
- Booth, Daniel G., Hood, Fiona E., Prior, Ian A., Royle, Stephen J., 2011. A TACC3/ch-TOG/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging. EMBO Journal, 30 (5), pp. 906-919
- Hood, F. E., Royle, Stephen J., 2009. Functional equivalence of the clathrin heavy chains CHC17 and CHC22 in endocytosis and mitosis. Journal of Cell Science, 122 (13), pp. 2185-2190
- Granseth, B., Odermatt, B., Royle, Stephen J., Lagnado, L., 2009. Comment on "The Dynamic Control of Kiss-and-Run and Vesicular Reuse Probed with Single Nanoparticles". Science, 325 (5947), pp. 1499-1499
- Royle, Stephen J., 2020. The digital cell : cell biology as a data science. New York, US, Cold Spring Harbor Laboratory Press
| Title | Funder | Award start | Award end |
|---|---|---|---|
| MRC Mid-range Equipment Application - January 2023 | MRC | 01 Aug 2023 | 31 Jul 2026 |
| CRUK Programme Grant | Cancer Research UK | 01 May 2019 | 30 Apr 2024 |
| MRC equipment full proposal | MRC | 01 Oct 2022 | 31 Mar 2023 |
| Role of TPD52-like proteins in cell migration and disease | MRC | 11 Sep 2017 | 10 Mar 2021 |
| Molecular mechanism of kinetochore fibre stabilisation - funding for years 4-6 | Cancer Research UK | 01 Feb 2016 | 31 May 2019 |
| Dr Royle Studentship: Molecular mechanisms of kinetochore fibre stabilisation | Cancer Research UK | 01 Jul 2013 | 30 Jun 2017 |
| Non-clinical PhD studentship: Regulation of podosome formation in vascular smooth muscle cells | British Heart Foundation | 01 Jan 2014 | 31 Dec 2016 |
| Molecular mechanisms of kinetochore fibre stabilisation - CRUK Fellowship | Cancer Research UK | 01 Feb 2013 | 31 Jan 2016 |
| A facility for 3D cellular imaging that bridges light and electron microscopy (BSRC ALERT 14 Equipment grant) | BBSRC | 21 Jan 2015 | 20 Jan 2016 |