Cloning TOPO cloning kit  Restriction Digest Restriction digest Digest your DNA with NEBcutter2 Ligation Protocol – Fermentas Troubleshooting ligation Competent cells Preparing chemically competent cells (Inoue) Method for making electorcompetent cells

Transformation Overview of E. coli genotypes Novagen competent cells protocol   E. coli strains TOP10F’  DH5alpha  BL21 Plasmid preparation – Fermentas Fast screening of transformants