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DNA protocols

PCR

PCR basics

Lego video filmbutton.jpg

What is PCR?

Troubleshooting

Troubleshooting Guide for PCR - Fermentas

Primer design

Primer design guidelines

Primer-BLAST - design software

Primer properties calculator

Purification of PCR fragments

Spin columns

Colony PCR filmbutton.jpg

PCR reagents

Instructions for making up 10 mM dNTPs

Quantification of DNA

DNA concentration and purity

Gel electrophoresis

Pouring a gel filmbutton.jpg

Running an electrophoresis gel filmbutton.jpg

1 kb ladder - Fermentas






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