Protein expression

Overview of Recombinant Protein Expression in E. coli

General overview protein synthesis – Piercenet

Antibiotic concentrations for E. coli selection

Recombinant protein expression in E. coli - EMBL

Advanced genetic strategies for recombinant protein expression in Escherichia coli. Sorensen and Mortensen (2005)

Recombinant protein expression in Escherichia coli. Baneyx (1999)

Cell lysis methods

Choice of lysis buffer and additives - EMBL

Preparation of cell lysates from E.coli - EMBL