Everything you need to know about protein purification

Protein fractionation

 Ammonium sulphate precipitation calculator

Tagged proteins

Overview of tag protein fusions

HisTrap columns

Ion exchange

How does ion exchange chromatography work?

Mono Q and Mono S

Gel filtration

Gel filtration animation

Superdex 200 10/300 GL 

PD-10 Desalting Column