WMS protocols/documents
Commonly-used Protocols
SDS Gel preparation and running
NOTE:
1) It is important that every strain/plasmid that you use in the lab is also kept as a glycerol stock.
2) This table is for long-term glycerol stocks. Maintain your own stocks separately as well. Do not take from these stocks unless absolutely required to reduce the number of freeze-thaw cycles.
3) When filling the glycerol stock inventory please do not leave any sections empty. Leave as much detail as possible.
Glycerol Stocks
Box A
Location |
Date
|
Strain |
Plasmid |
Source |
Remarks |
Antibiotics |
Added by |
A1 |
8/2/2017 |
BL21(DE3) |
pWaldo |
Liz Fullam |
GFP |
KAN |
Muhammad |
B1 |
9/3/2017 |
TOP10 |
No plasmid |
Liz Fullam |
- |
STR |
Muhammad |
C1 |
9/3/2017 |
TOP10 |
No plasmid |
Liz Fullam |
- |
STR |
Muhammad |
D1 |
9/3/2017 |
BL21(DE3) |
No plasmid |
Liz Fullam |
- |
None |
Muhammad |
E1 |
9/3/2017 |
BL21(DE3) |
No plasmid |
Liz Fullam |
- |
None |
Muhammad |
F1 |
24/2/2017 |
TOP10 |
pET-29a(+) |
Nick Waterfield |
w/o insert |
KAN |
Julia |
G1 |
24/2/2017 |
TOP10 |
pET-29a(+) |
Nick Waterfield |
w/o insert |
KAN |
Julia |
H1 |
7/6/2017 |
CodonPlus RIPL |
No plasmid |
Liz Fullam |
- |
CAM, STR |
Muhammad |
I1 |
8/6/2017 |
CodonPlus RIPL |
No plasmid |
Liz Fullam |
- |
CAM, STR |
Muhammad |
A2 |
9/3/2017 |
Rosetta |
No plasmid |
David Roper |
- |
CAM |
Muhammad |
B2 |
9/3/2017 |
Rosetta |
No plasmid |
David Roper |
- |
CAM |
Muhammad |
C2 |
9/3/2017 |
TOP10 |
pET20b |
Peter Davies |
AFP III |
AMP |
Muhammad |
D2 |
9/3/2017 |
BL21 |
pET20b |
Peter Davies |
AFP III |
AMP |
Muhammad |
E2 |
30/3/2017 |
TOP10 |
pET24a |
Peter Davies |
Maxi |
KAN, GEN |
Muhammad |
F2 |
30/3/2017 |
M. Smegmatis |
No plasmid |
Liz Fullam |
- |
AMP |
Muhammad |
G2 |
19/6/2017 |
Bacillus |
No plasmid |
Emma Denham |
- |
None |
Muhammad |
H2 |
19/6/2017 |
Rosetta |
pET32a |
Own stock |
AFPI |
CAM, AMP |
Muhammad |
I2 |
19/6/2017 |
CodonPlus RIPL |
pET32a |
Own stock |
AFPI |
CAM, AMP |
Muhammad |
A3 |
19/6/2017 |
TOP10 |
pET32a |
Own stock |
AFPI |
AMP |
Muhammad |
B3 |
19/6/2017 |
CodonPlus RIPL |
pET21a |
Kelley Moremen |
GFP-TEV |
KAN |
Muhammad |
C3 |
19/6/2017 |
BL21 (PlysS) |
pET151/D-TOPO |
Ann Dixon |
PDGFR20 |
AMP |
Muhammad |
D3 |
22/7/2017 |
TOP10 |
pWaldo |
Liz Fullam |
GFP |
KAN |
Julia |
E3 |
22/7/2017 |
BL21 |
pWaldo |
Liz Fullam |
GFP |
KAN |
Julia |
F3 |
22/7/2017 |
TOP10 |
pET21b |
Nick Waterfield |
w/o insert |
AMP |
Julia |
G3 |
22/7/2017 |
TOP10 |
pET21b |
Nick Waterfield |
PETase |
AMP |
Julia |
H3 |
2/8/2017 |
CodonPlus RIPL |
pET21b |
Nick Waterfield |
PETase |
AMP, CAM, STR |
Julia |
I3 |
04/08/2017 |
TOP10 |
pSNAP-tag (T7)-2 |
Own stock |
AFPIII-SNAP |
AMP |
Muhammad |
4A |
04/08/2017 |
BL21 |
pSNAP-tag (T7)-2 |
Own stock |
AFPIII-SNAP |
AMP |
Muhammad |
4B |
04/08/2017 |
TOP10 |
pET20b |
Own stock |
AFPIII-Cys |
AMP |
Muhammad |
4C |
04/08/2017 |
BL21 |
pET20b |
Own stock |
AFPIII-Cys |
AMP |
Muhammad |
4D |
12/12/2017 |
BL21 |
pET21a |
Kelley Moremen |
GFP-TEV |
AMP |
Iain |
4E |
09/04/2018 |
P. agglomerans |
- |
NBRC (Japan) |
- |
- |
Muhammad |
4F |
09/04/2018 |
P. agglomerans |
- |
NBRC (Japan) |
- |
- |
Muhammad |
4G |
09/04/2018 |
P. agglomerans |
- |
NBRC (Japan) |
- |
- |
Muhammad |