Profile

Chemical engineer with a PhD. in chemistry, highly skilled in analysis of complex samples (proteins, oil and DNA-metal complexes) mainly by Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS, Bruker solariX), achieving sub-ppm mass accuracy. Experience in dissociation methods by mass spectrometry to achieve reaction sites and chemical structure identification. Proficient in analysis of spectra using a range of software packages. Background in implementation of ISO/IEC 17025 and good laboratory practices.

Education

2009-present University of Warwick, United Kingdom

PhD in chemistry, warwick centre for analytical science studentship, with Prof. Peter O’Connor. I studied the glyoxal-glycation binding site on peptides and proteins using an FT-ICR-MS. The resulting data is analyzed manually or by use of home-built MATLAB script.

1992-1999 National University of Colombia, Colombia

B.Sc. chemical engineering, project on production and quality control for the food industry.

Summary of Key Research Techniques

Ionization methods: Electrospray ionization (ESI), nano-electrospray ionization, atmospheric pressure photoionization (APPI), atmospheric pressure chemical ionization (APCI), matrix assisted laser desorption/ionization (MALDI).

Mass spectrometers used: FT-ICR, time of flight (TOF), ion trap, and inductively coupled plasma (ICP) mass spectrometers.

Chromatography methods: nano-liquid chromatography and gas chromatography coupled with mass spectrometry.

Dissociation methods: Electron capture dissociation (ECD), collisionally activated dissociation, electron transfer dissociation, double resonance-ECD, infrared multiphoton dissociation (IRMPD).

Software: MATLAB, DataAnalysis 4.0 (Bruker Daltonics), Origin, LateX, Amber and Gaussian.

Professional Experience

2009-PRESENT FOURIER TRANSFORM ION CYCLOTRON RESONANCE LABORATORY, DEPARTMENT OF CHEMISTRY, UNIVERSITY OF WARWICK, COVENTRY, UNITED KINGDOM

Operation of 12 tesla FT-ICR-MS, Bruker solariX, including sample preparation, tuning, optimization, calibration and data analysis.

Cryogens fills for superconducting magnet.

Close working relationship with Bruker Daltonics, United Kingdom.

Supervision and training of project students.

2005-2008 SGS Colombia, PROFESSIONAL SERVICES-FREE LANCE

Providing training for internal audits under ISO/IEC 17025:2005, ISO/IEC 17024 standards and for courses in Good Laboratory Practice (GLP).

Conducting external audits to laboratories according to ISO/IEC 17025:2005 standard.

Designing sampling plans, leading technical inspections and analyzing results for product certification.

Providing consultancy services to laboratories in accreditation process by ISO/IEC 17025:2005 standard, including: diagnostic audit, making administrative and technical procedures and implementation of control procedures, calibration, validation and uncertainty calculus.

Designing sampling plans, quality control program for process control and design of the quality control laboratory for a bio-diesel plant producer.

2000-2005 NUTRIANALISIS LTDA, PROFESSIONAL IN ANALYTICAL CHEMISTRY

Doing physicochemical and instrumental analyses to drinking water and wastewater.

Performing and standardizing analytical tests for food and feedstuffs by high performance liquid chromatography (HPLC).

Supervising and training new laboratory technicians.

Performing as the laboratory’s representative for National Technical Committees.

Helping the external adviser in the implementation of the ISO/IEC 17025:2000 standard, writing technical procedures and analyzing the root cause to nonconformities to take corrective actions.

Successful Fundraising and publications

Warwick-Santander Universities Agreement: Latin America Collaboration Awards, Santander Research Grant Fund. Coventry, UK. December 2010.

Lopez-Clavijo, A. F.; Barrow, M. P.; Rabbani, N.; Thornalley, P. J.; O'Connor, P. B. Determination of types and binding sites of Advanced Glycation Endproducts for Substance P. Analytical Chemistry. 2012 (84): 10568-10575.

Lopez-Clavijo, A. F.; Duque-Daza, C. A.; P. J.; O'Connor, P. B. Tandem mass spectrometry for the study of glyoxal-derived advanced glycation end-products (AGEs) in peptides. Rapid Communications in Mass Spectrometry 2014 (28):25-32.

Lopez-Clavijo, A. F.; Barrow, M. P.; Kilgour, D.; Rabbani, N.; Thornalley, P. J.; O'Connor, P. B. Study of an Unusual Advanced Glycation End-product (AGE) Derived from Glyoxal using Mass Spectrometry. Submitted. 2013.

Lopez-Clavijo, A. F.; Duque-Daza, C. A.; Soulby, A.; P. J.; O'Connor, P. B. Unexpected Crosslinking and Diglycation as Advanced Glycation End-products (AGEs). Submitted. 2013.

Lopez-Clavijo, A. F.; Duque-Daza, C. A.; P. J.; O'Connor, P. B. A decade of electron capture dissociation.Submitted. 2013.

Recent Conferences

Oral presentation: “ Glycation of Substance P” at “British Mass Spectrometry Society” meeting. Cheshire, UK. April 2012.

Oral presentation: “Glycation of Substance P” at the 10^thEuropean Fourier Transform Mass Spectrometry Workshop. Coventry, UK. April 2012.

Poster presentation: “Glyoxal binding site identification in peptides by ECD and CAD using FT-ICR-MS” at “British Mass Spectrometry Society” meeting. Cardiff, UK. September 2011.

Attended the 9^thEuropean FT-ICR-MS workshop. Switzerland, April 2010.

Teaching / Lecturing Experience

Demonstrator for chemistry, maths and physics for chemist, and physical chemistry during my PhD.

Design, preparation and teaching of the course for internal auditor ISO/IEC 17025 and ISO/IEC 17024at “SGS Colombia.”< /font> Bogota-Cundinamarca, Piedecuesta-Santander, Cartagena-Bolivar, Cali-Valle, Pereira-Risaralda. Colombia. Medellin-Antioquia. Course, held for five days, taught in multiple occasions during 2006 and 2007.

Design, preparation and teaching of the course on good laboratory practices (GLP) at “SGS Colombia.” Bogota, Colombia. Course, held for two days, taught in multiple occasions during 2006 and 2007.

Training and Development

Attended course on Amber software package at the Imperial College of London, United Kingdom, 2012.

Attended the training course of Internal Auditor in Good Manufacturing Practices (GMP), and Hazard Analysis and Critical Control Points (HACCP), SGS Colombia, Bogotá, Colombia, 2005.

Attended the training course of Internal Auditor in ISO/IEC 17025, General requirements for the competence of testing and calibration laboratories, SGS Colombia, Bogotá, Colombia, 2004.

Internship at the quality control laboratory ICTA-National University of Colombia, 1998.

References

Dr. Mark P. Barrow. Department of Chemistry, University of Warwick,, Coventry, United Kingdom. e-mail: M.P.Barrow@warwick.ac.uk

Prof. Peter B. O’C onnor. Department of Chemistry, University of Warwick,, Coventry, United Kingdom. e-mail: p.oconnor@warwick.ac.uk

Iván R. Rojas.Product Manager, Systems Certification SGS Colombia, Bogotá, Colombia. e-mail: ivan.rojas@sgs.com