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Chemistry Peter O'Connor Group

Federico Floris

Ph.D., Analytical Chemistry (Fourier transform ion cyclotron resonance mass spectrometry, FT-ICR MS)

Pushing the limits of 2D FT-ICR MS.


  • Development of a method for the determination of macromolecules in complex matrices through the use of Two-dimensional (2D) FT-ICR MS.
  • Application of 2D MS to Top-down/Bottom-up Proteomics, polymer analysis, Glycomics.
  • Construction and improvement of a Fourier Transform Ion Cyclotron Resonance (FT-ICR) mass spectrometer.
  • Orchestrated laboratory maintenance, safety, and reagents and other materials stock control.
  • Responsible for the maintenance of a GC-MS for teaching and demonstrating purposes.
  • Taught a GC-MS laboratory course to undergraduate and master students. Tutored students during their summer projects involving FT-ICR MS. Trained new Ph.D. students in laboratory safety and maintenance.
  • Developed confidence in talking in public, and a strong visual sense for proportions and order in graphics.

I actively promote awareness in the working environment about mental health, gender equality, LGBTQI+ rights, and cystic fibrosis.

Publications

Floris, F. and O’Connor, P.B. Fundamentals of 2D FT-MS. In Fundamentals and Applications of Fourier Transform Mass Spectrometry; Schmitt-Kopplin, P. and Kanawati, B, Ed.; Elsevier, 2018. Chapter submitted.

Floris, F.; Chiron L.; Delsuc M.-A.; Barrow P.M.; O'Connor, P.B. (2018): Top-Down Deep Sequencing of Ubiquitin using Two-dimensional Mass Spectrometry. Anal. Chem. https://pubs.acs.org/doi/abs/10.1021/acs.analchem.8b00500

Floris, F.; Chiron L.; Delsuc M.-A.; Barrow P.M.; O'Connor, P.B. (2018): Application of Tandem Two-dimensional Mass Spectrometry for Deep Sequencing of Calmodulin. J. Am. Soc. Mass Spectrom. https://doi.org/10.1007/s13361-018-1978-y

Floris, F.; van Aghtoven, M.A.; Chiron L.; Wootton A.C.; Lam P.Y.; Delsuc M.-A.; Barrow P.M.; O'Connor, P.B. (2017): Bottom-up two-dimensional electron-capture dissociation mass spectrometry of calmodulin. J. Am. Soc. Mass Spectrom. DOI: 10.1007/s13361-017-1812-y

Floris, F.; Vallotto, C.; Chiron L.; Lynch, A.; Delsuc M.-A.; Barrow P.M.; O'Connor, P.B. (2017): Polymer analysis in the second dimension: preliminary studies for the characterization of polymers with 2D MS. Analytical Chemistry 89 (18), 9892-9899. DOI: 10.1021/acs.analchem.7b02086

Floris, F.; van Aghtoven, M.A.; Chiron L.; Soulby, A.; Wootton A.C.; Lam P.Y.; Delsuc M.-A.; Barrow P.M.; O'Connor, P.B. (2016): Two-dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometry of Calmodulin: a top-down and bottom-up approach. J. Am. Soc. Mass Spectrom. 27(9), 1531–1538. DOI: 10.1007/s13361-016-1431-z

Simon, H., van Agthoven, M.A., Lam, P.Y., Floris, F., Chiron, L., Delsuc, M.-A., Rolando, C., Barrow, M., O’Connor, P. (2015): Uncoiling Collagen: a multidimensional mass spectrometry study. The Analyst 141, 157-165. DOI:10.1039/C5AN01757B

Saeed A.,1 Floris F.,1 Andersson U., Pikuleva I., Lövgren-Sandblom A., Bjerke M., Paucar M., Wallin A., Svenningsson P., Björkhem I., “7α-hydroxy-3-oxo-4-cholestenoic acid in cerebrospinal fluid reflects the integrity of the blood-brain barrier.” J Lipid Res. 2014 Feb; 55(2):313-8. 1Authors with equal contribution.

Presentations

Oral presentations

2017/12- Floris, F; van Agthoven, M; Chiron, L; Lynch M.A.; Lam P.Y.; Simon, H; Wootton, C; Gleinich, A.; Barrow, MP; Delsuc, MA; O'Connor, PB; 2D FT-ICR MS: two dimensions, infinite possibilities. R&D Topics 2017, Hobart University, Tasmania (Australia).

2017/05 - Floris, F; van Agthoven, M; Chiron, L; Lynch M.A.; Lam P.Y.; Simon, H; Wootton, C; Gleinich, A.; Barrow, MP; Delsuc, MA; O'Connor, PB; 2D FT-ICR MS: two dimensions, infinite possibilities. Warwick Chemistry Symposium 2017, University of Warwick (UK).

2016/09 - Floris, F; van Agthoven, M; Chiron, L; Lynch M.A.; Lam P.Y.; Simon, H; Wootton, C; Barrow, MP; Delsuc, MA; Rolando, C; O'Connor, PB; 2D FT-ICR MS for Top-down protein analysis. British Mass Spectometry Society, BMSS Annual Meeting, Eastbourne (UK).

2016/04 - Floris, F; van Agthoven, M; Chiron, L; Soulby, A; Wootton, C; Lam P.Y.; Lynch M.A.; Barrow, MP; Delsuc, MA; Rolando, C; O'Connor, PB; Top-down analysis of Calmodulin using 2D FT-ICR MS. European Fourier Transform Mass Spectrometry, EFTMS 2016, Matera (Italy). Download

2015/11 - Floris, F; van Agthoven, M; Chiron, L; Soulby, A; Wootton, C; Lam P.Y.; Barrow, MP; Delsuc, MA; Rolando, C; O'Connor, PB; Two-dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometry of Calmodulin: a top-down and bottom-up approach. 14th East Midlands Proteomics Workshop, University of Warwick, Coventry (UK).

Posters

FF_Poster_ASMS20152015/06 - Floris, F; van Agthoven, M; Chiron, L Soulby, A; Wootton, C; Barrow, MP; Delsuc, MA; Rolando, C; O'Connor, PB; Two-dimensional Fourier Transform Ion Cyclotron Resonance Mass Spectrometry of Calmodulin: a top-down and bottom-up approach, 63rd conference of American Society of Mass Spectrometry, St. Louis, Missouri (USA).

2016/09 - Floris, F; van Agthoven, M; Chiron, L Soulby, A; Wootton, C; Lam, P.Y; Barrow, MP; Delsuc, MA; O'Connor, PB; Analitica2016, Giardini Naxos, Messina (Italy).

2016/06 - Floris, F; van Agthoven, M; Chiron, L Soulby, A; Wootton, C; Lam, P.Y; Barrow, MP; Delsuc, MA; O'Connor, PB; 64th conference of American Society of Mass Spectrometry, San Antonio, Texas (USA)

2016/01 - Floris, F; van Agthoven, M; Chiron, L Soulby, A; Wootton, C; Barrow, MP; Delsuc, MA; O'Connor, PB; LGBT STEMinary, University of Sheffield (UK).

2015/10 - Floris, F; van Agthoven, M; Chiron, L Soulby, A; Wootton, C; Barrow, MP; Delsuc, MA; O'Connor, PB; Biophysics: measuring and modeling biology, Les Houches (France).

2015/09 - Floris, F; van Agthoven, M; Chiron, L Soulby, A; Wootton, C; Barrow, MP; Delsuc, MA; O'Connor, PB; BMSS Annual Meeting, University of Birmingham (UK).

Awards

  • 2016 - BMSS travel grant to present my research at a national conference (BMSS annual meeting 2016).
  • 2016 – Australian Bicentennial Scholarships and Fellowships Award to finance a project in collaboration with Monash University (Melbourne, Australia).
  • 2016 – Italian Chemical Society (SCI) Fellowship to present my research at the Analytical Chemistry conference Analitica2016 in Messina (Italy).
  • 2016 – Italian Chemical Society (SCI) Fellowship to present my research at the 12th EFTMS workshop and the 1st EFTMS School in Matera (Italy).
  • 2015 – Warwick Transatlantic Fellowship to finance a short project in collaboration with the University of California, Los Angeles.
  • 2015 – BMSS travel grant to present my research at an international conference (63rd ASMS conference).
  • 2013 – Leonardo da Vinci fellowship winner (7th over 84 winners) for a six-month internship in Unilever Port Sunlight, UK, promoted by University of Rome La Sapienza.

@ChemFloris

ORCID iD iconorcid.org/0000-0001-6901-6308

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Department of Chemistry
The University of Warwick
Millburn Hill Road
Coventry CV4 7AL, UK
Office: +44 (0) 24 7615 1304
Lab: +44 (0) 24 7615 1305
Email: F dot Floris at warwick dot ac dot uk

Warwick LGBT+ Role Models Guide

Warwick LGBTUA+ Role Models Guide

ORCID