Latest Publications
Detection of membrane fission in single Bacillus subtilis cells during endospore formation with high temporal resolution
Ane Landajuela, Martha Braun, Christopher D.A. Rodrigues, Erdem Karatekin
Membrane fission is an essential process in all domains of life. The underlying mechanisms remain poorly understood in bacteria, partly because suitable assays are lacking. Here, we describe an assay to detect membrane fission during endospore formation in single Bacillus subtilis cells with a temporal resolution of ∼1 min. Other cellular processes can be quantified and temporally aligned to the membrane fission event in individual cells, revealing correlations and causal relationships.