Johannes Boltze publications
Development of a routinely applicable imaging protocol for fast and precise middle cerebral artery occlusion assessment and perfusion deficit measure in an ovine stroke model: a case study
Herrmann AM, Cattaneo GFM, Eiden SA, Wieser M, Kellner E, Maurer C, Haberstroh J, Mulling C, Niesen WD, Urbach H, Boltze J, Meckel S, Shah MJ
Temporary middle cerebral artery occlusion (MCAO) in sheep allows modeling of acute large vessel occlusion stroke and subsequent vessel recanalization. However, rapid and precise imaging-based assessment of vessel occlusion and the resulting perfusion deficit during MCAO still represents an experimental challenge. Here, we tested feasibility and suitability of a strategy for MCAO verification and perfusion deficit assessment. The results underline the need for intra-ischemic perfusion assessment and future studies focusing on the correlation between perfusion deficit, MCAO duration, and final infarct volume. Temporary MCAO and intra-ischemic perfusion imaging nevertheless has the potential to be applied for the simulation of novel recanalization therapies.
Clinical practice guidelines of remote ischemic conditioning for the management of cerebrovascular diseases
Biochemical characterization of Serpula lacrymans iron-reductase enzymes in lignocellulose breakdown
Irnia Nurika, Daniel C Eastwood, Timothy DH Bugg, Guy C Barker
Putative iron-reductase (IR) genes from Serpula lacrymans with similarity to the conserved iron-binding domains of cellobiose dehydrogenase (CDH) enzymes have been identified. These genes were cloned and expressed to functionally characterize their activity and role in the decomposition of lignocellulose. The results suggest that both IR1 and IR2 enzymes mediate a non-enzymatic depolymerisation of lignocellulose and highlight the potential of chelator-mediated Fenton systems in the industrial pre-treatment of biomass.
Dimeric benzoboroxoles for targeted activity against Mycobacterium tuberculosis
Collette S Guy, Kathryn Murray, Matthew I Gibson and Elizabeth Fullam
Dimeric benzoboroxoles that are covalently linked by a short scaffold enhance selective anti-tubercular activity. These multimeric benzoboroxole compounds are capable of engaging the specific extracellular Mycobacterium tuberculosis glycans, do not lead to the evolution of resistance and bypass the need to cross the impermeable mycobacterial cell envelope barrier.
Novel clinically relevant antibiotic resistance genes associated with sewage sludge and industrial waste streams revealed by functional metagenomic screening
L Zhang, L Calvo-Bado, AK Murray, GCA Amos, PM Hawkey, EM Wellington, WH Gaze
A growing body of evidence indicates that anthropogenic activities can result in increased prevalence of antimicrobial resistance genes (ARGs) in bacteria in natural environments. Characterization of ARGs in environmental metagenomes is important for understanding the evolution and dissemination of resistance, as there are several examples of clinically important resistance genes originating in environmental species. This work confirms the natural environment as a reservoir of novel and mobilizable resistance genes, which may pose a threat to human and animal health.
Diurnal Expression of Arabidopsis Gene Homologs during Daylength-Regulated Bulb
Md. Harun Ar Rashid, Brian Thomas
Bulb initiation in long-day onion is regulated at the physiological level in a similar way to the photoperiodic regulation of flowering in Arabidopsis. This study establishes the diurnal time-course expression, in onion, of key genes particularly linked to circadian regulation in Arabidopsis. These findings will help to understand the basis of the difference between responses of onions adapted to different latitudes, which is important for developing new varieties.
Identification and QTL mapping of resistance to Turnip yellows virus (TuYV) in oilseed rape, Brassica napus
Dieter Hackenberg, Elvis Asare‑Bediako. Adam Baker, Peter Walley, Carol Jenner, Shannon Greer, Lawrence Bramham, Jacqueline Batley, David Edwards, Regine Delourme, Guy Barker, GrahamTeakle, John Walsh
Turnip yellows virus (TuYV) is transmitted by the peach-potato aphid (Myzus persicae) and causes severe yield losses in commercial oilseed rape crops (Brassica napus). There is currently only one genetic resource for resistance to TuYV available in brassica, which was identified in the re-synthesised B. napus line ‘R54’. In our study, 27 mostly homozygous B. napus accessions, representing a diverse subset of the B. napus genepool, were screened for TuYV resistance/susceptibility. This is the first report of the QTL mapping of TuYV resistance in natural B. napus.