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Latest Publications

Phill Stansfeld publications

Cell dedc19In situ structure of an intact lipopolysaccharide-bound bacterial surface layer

Andriko von Kügelgen, Haiping Tang, Gail G Hardy, Danguole Kureisaite-Ciziene, Yves V Brun, Phillip J Stansfeld, Carol V Robinson, and Tanmay A M. Bharat

Most bacterial and all archaeal cells are encapsulated by a paracrystalline, protective, and cell-shape-determining proteinaceous surface layer (S-layer). On Gram-negative bacteria, S-layers are anchored to cells via lipopolysaccharide. Here, we report an electron cryomicroscopy structure of the Caulobacter crescentus S-layer bound to the O-antigen of lipopolysaccharide. A complete atomic structure of the S-layer shows the power of cellular tomography for in situ structural biology and sheds light on a very abundant class of self-assembling molecules with important roles in prokaryotic physiology with marked potential for synthetic biology and surface-display applications.

Cell. December 2019

Angewandte ChemieA mass spectrometry-based approach to distinguish annular and specific lipid binding to membrane proteins

Jani Reddy Bolla, Robin A Corey, Cagla Sahin, Joseph Gault, Alissa Hummer, Jonathan TS Hopper, David P Lane, David Drew, Timothy M Allison, Phillip J Stansfeld, Carol V Robinson and Michael landreh

Membrane proteins engage in a variety of contacts with their surrounding lipids, but distinguishing between specifically boundlipids, and non‐specific annular interactionsis a challenging problem. Applying native mass spectrometry to three membrane protein complexes with different lipid binding properties, we explore the ability of detergents to compete with lipids bound in different environments. In summary, we distinguish annular from non‐annular lipids based on their exchange rates in solution.

Angewandte Chemie. Dec 2019

Wed 15 Jan 2020, 09:01

TGFB-INHB/activin signaling regulates age-dependent autophagy and cardiac health through inhibition of MTORC2 Autophagy

autophagyKai Chang, Ping Kang, Ying Liu, Kerui Huang, Ting Miao, Erika Taylor, Antonia P Sagona, Ioannis P Nezis, Rolf Bodmer, Karen Ocorr, Hua Bai

Age-related impairment of macroautophagy/autophagy and loss of cardiac tissue homeostasis contribute significantly to cardiovascular diseases later in life. MTOR (mechanistic target of rapamycin kinase) signaling is the most well-known regulator of autophagy, cellular homeostasis, and longevity. The MTOR signaling consists of two structurally and functionally distinct multiprotein complexes, MTORC1 and MTORC2. While MTORC1 is well characterized but the role of MTORC2 in aging and autophagy remains poorly understood. Here we identified TGFB-INHB/activin signaling as a novel upstream regulator of MTORC2 to control autophagy and cardiac health during aging. , Our studies discover the TGFB-INHB/activin-mediated inhibition of TORC2 as a novel mechanism for age-dependent decreases in autophagic activity and cardiac health.

Autophagy. December 2019

Mon 13 Jan 2020, 08:15 | Tags: Biomedical Science

NCAM Regulates Temporal Specification of Neural Progenitor Cells via Profilin2 during Corticogenesis

Journal of Cell BiologyHuang R, Yuan DJ, Li S, Liang XS, Gao Y, Lan XY, Qin HM, Ma YF, Xu GY, Schachner M, Sytnyk V, Boltze J, Ma QH, Li S

The development of cerebral cortex requires spatially and temporally orchestrated proliferation, migration, and differentiation of neural progenitor cells (NPCs). The molecular mechanisms underlying cortical development are, however, not fully understood. The neural cell adhesion molecule (NCAM) has been suggested to play a role in corticogenesis. Here we show that NCAM is dynamically expressed in the developing cortex. The results show that NCAM plays an essential role in NPC proliferation and fate decision during cortical development by regulating profilin2-dependent actin polymerization.

Journal of Cell Biology. December 2019

Fri 10 Jan 2020, 10:44

Nano-encapsulated Escherichia coli Divisome Anchor ZipA, and in complex with ftsZ

Scientific ReportsSarah C Lee, Richard Collins, Yu-Pin Lin, Mohammed Jamshad, Claire Broughton, Sarah A Harris, Benjamin S Hanson, Cecilia Tognoloni, Rosemary A Parslow, Ann E Terry, Alison Rodger, Corinne J Smith, Karen J Edler, Robert Ford, David I Roper & Timothy R Dafforn

The E. coli membrane protein ZipA, binds to the tubulin homologue ftsZ, in the early stage of cell division. We isolated ZipA in a Styrene Maleic Acid lipid particle (SMALp) preserving its position and integrity with native E. coli membrane lipids. Direct binding of ZipA to ftsZ is demonstrated, including FtsZ fibre bundles decorated with ZipA. The complex extends 12 nm from the membrane in a compact structure, supported by mesoscale modelling techniques, measuring the movement and stiffness of the regions within ZipA provides molecular scale analysis and visualisation of the early divisome.

Scientific Reports. December 2019

Wed 08 Jan 2020, 10:58 | Tags: Biomedical Science

Transcriptome and organellar sequencing highlights the complex origin and diversification of alloteraploid Brassica napus

 Nature Communications dec19Hong An, Xinshuai Qi, Michelle L. Gaynor, Yue Hao, Sarah C. Gebken, Makenzie E. Mabry, Alex C. McAlvay, Graham R. Teakle, Gavin C. Conant, Michael S. Barker, Tingdong Fu, Bin Yi, and J. Chris Pires

Brassica napus, an allotetraploid crop, is hypothesized to be a hybrid from unknown varieties of Brassica rapa and Brassica oleracea. Despite the economic importance of B. napus, much is unresolved regarding its phylogenomic relationships, genetic structure, and diversification. Here we conduct a comprehensive study among diverse accessions from 183 B. napus (including rapeseed, rutabaga, and Siberian kale), 112 B. rapa, and 62 B. oleracea and its wild relatives. This study highlights the complex origin and evolution of B. napus providing insights that can further facilitate B. napus breeding and germplasm preservation.

Nature Communications.December 2019

Tue 07 Jan 2020, 13:04

Genome sequences of human coronavirus OC43 and NL63 associated with respiratory Infections in Kilifi, Kenya

Microb Resource Announcements dec19Everlyn Kamau, Martha M Luka, Zaydah R de Laurent, Irene Adema, Charles N Agoti and D James Nokes

Coding-complete genomes of two human coronavirus OC43 strains and one NL63 strain were obtained by metagenomic sequencing of clinical samples collected in 2017 and 2018 in Kilifi, Kenya. Maximum likelihood phylogenies showed that the OC43 strains were genetically dissimilar and that the NL63 strain was closely related to NL63 genotype B viruses

Microbiology Resource Announcements. December 2019

Fri 20 Dec 2019, 08:04 | Tags: Biomedical Science

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