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Johannes Boltze publications

Frontiers in NeurologyDevelopment of a routinely applicable imaging protocol for fast and precise middle cerebral artery occlusion assessment and perfusion deficit measure in an ovine stroke model: a case study

Herrmann AM, Cattaneo GFM, Eiden SA, Wieser M, Kellner E, Maurer C, Haberstroh J, Mulling C, Niesen WD, Urbach H, Boltze J, Meckel S, Shah MJ

Temporary middle cerebral artery occlusion (MCAO) in sheep allows modeling of acute large vessel occlusion stroke and subsequent vessel recanalization. However, rapid and precise imaging-based assessment of vessel occlusion and the resulting perfusion deficit during MCAO still represents an experimental challenge. Here, we tested feasibility and suitability of a strategy for MCAO verification and perfusion deficit assessment. The results underline the need for intra-ischemic perfusion assessment and future studies focusing on the correlation between perfusion deficit, MCAO duration, and final infarct volume. Temporary MCAO and intra-ischemic perfusion imaging nevertheless has the potential to be applied for the simulation of novel recanalization therapies.

Frontiers in Neurology. November 2019

Conditioning MedicineClinical practice guidelines of remote ischemic conditioning for the management of cerebrovascular diseases

Xunming Ji, Wenbo Zhao, Johannes Boltze, Sijie Li, Ran Meng, Yuan Wang, Gregory J Bix, Cesar V Borlongan, Jeffrey M Gidday, Sebastian Koch, John C Quindry, Rajiv R Ratan, Kristin Veighey, Guohua Xi, Giuseppe Pignataro, David C Hess, and Derek J Hausenloy J

Remote ischemic conditioning (RIC) using transient limb ischemia and reperfusion has been shown in small clinical studies to reduce myocardial injury and infarction in cardiac patients. Beneficial effects of limb RIC were also reported in a number of different settings of cerebrovascular disease. Although limb RIC has many advantages, several questions remain regarding its clinical application for cerebrovascular disease. Therefore, in this document, we aim to provide practicing clinicians with a coherent synthesis of the latest scientific evidence, and we propose several recommendations to help facilitate the clinical application of limb RIC for the management of cerebrovascular disease. Conditioning Medicine. November 2019

Fri 06 Dec 2019, 08:32 | Tags: Biomedical Science

Biochemical characterization of Serpula lacrymans iron-reductase enzymes in lignocellulose breakdown

J.Ind.Microbio&Biotech nov19.jpgIrnia Nurika, Daniel C Eastwood, Timothy DH Bugg, Guy C Barker

Putative iron-reductase (IR) genes from Serpula lacrymans with similarity to the conserved iron-binding domains of cellobiose dehydrogenase (CDH) enzymes have been identified. These genes were cloned and expressed to functionally characterize their activity and role in the decomposition of lignocellulose. The results suggest that both IR1 and IR2 enzymes mediate a non-enzymatic depolymerisation of lignocellulose and highlight the potential of chelator-mediated Fenton systems in the industrial pre-treatment of biomass.

Journal of Industrial Microbiology & Biotechnology. November 2019

Wed 04 Dec 2019, 07:55

Dimeric benzoboroxoles for targeted activity against Mycobacterium tuberculosis

Organic & Biomolecular Chemistry nov19Collette S Guy, Kathryn Murray, Matthew I Gibson and Elizabeth Fullam

Dimeric benzoboroxoles that are covalently linked by a short scaffold enhance selective anti-tubercular activity. These multimeric benzoboroxole compounds are capable of engaging the specific extracellular Mycobacterium tuberculosis glycans, do not lead to the evolution of resistance and bypass the need to cross the impermeable mycobacterial cell envelope barrier.

Organic & Biomolecular Chemistry. November 2019

Mon 02 Dec 2019, 08:18 | Tags: Biomedical Science

Novel clinically relevant antibiotic resistance genes associated with sewage sludge and industrial waste streams revealed by functional metagenomic screening

Environment International nov19L Zhang, L Calvo-Bado, AK Murray, GCA Amos, PM Hawkey, EM Wellington, WH Gaze

A growing body of evidence indicates that anthropogenic activities can result in increased prevalence of antimicrobial resistance genes (ARGs) in bacteria in natural environments. Characterization of ARGs in environmental metagenomes is important for understanding the evolution and dissemination of resistance, as there are several examples of clinically important resistance genes originating in environmental species. This work confirms the natural environment as a reservoir of novel and mobilizable resistance genes, which may pose a threat to human and animal health.

Environment International. November 2019

Fri 29 Nov 2019, 08:19 | Tags: Environmental Bioscience

Diurnal Expression of Arabidopsis Gene Homologs during Daylength-Regulated Bulb

Scientia Horticulturae nov19Md. Harun Ar Rashid, Brian Thomas

Bulb initiation in long-day onion is regulated at the physiological level in a similar way to the photoperiodic regulation of flowering in Arabidopsis. This study establishes the diurnal time-course expression, in onion, of key genes particularly linked to circadian regulation in Arabidopsis. These findings will help to understand the basis of the difference between responses of onions adapted to different latitudes, which is important for developing new varieties.

Scientia Horticulturae. November 2019

Thu 28 Nov 2019, 08:14 | Tags: Plant and Crop Science

Identification and QTL mapping of resistance to Turnip yellows virus (TuYV) in oilseed rape, Brassica napus

Theoretical & Applied Genetics nov19Dieter Hackenberg, Elvis Asare‑Bediako. Adam Baker, Peter Walley, Carol Jenner, Shannon Greer, Lawrence Bramham, Jacqueline Batley, David Edwards, Regine Delourme, Guy Barker, GrahamTeakle, John Walsh

Turnip yellows virus (TuYV) is transmitted by the peach-potato aphid (Myzus persicae) and causes severe yield losses in commercial oilseed rape crops (Brassica napus). There is currently only one genetic resource for resistance to TuYV available in brassica, which was identified in the re-synthesised B. napus line ‘R54’. In our study, 27 mostly homozygous B. napus accessions, representing a diverse subset of the B. napus genepool, were screened for TuYV resistance/susceptibility. This is the first report of the QTL mapping of TuYV resistance in natural B. napus.

Theoretical and Applied Genetics November 2019

Mon 25 Nov 2019, 08:45 | Tags: Plant and Crop Science

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