Characterisation of a novel method for the production of single-span membrane proteins in Escherichia coli
Smith S, Walker K, Jones A, Smith C, Robinson C
The large‐scale production and isolation of recombinant protein is a central element of the biotechnology industry and many of the products have proved extremely beneficial for therapeutic medicine. Escherichia coli is the microorganism of choice for the expression of heterologous proteins for therapeutic application, and a range of high‐value proteins have been targeted to the periplasm using the well characterised Sec protein export pathway. The data presented here demonstrate a novel method for the production of single‐span membrane proteins in E. coli.