Tapestation & Bioanalyzer and Qubit Service
Agilent Tapestation 4150
The Agilent TapeStation is an automated electrophoresis system used for the quality control (QC) of DNA and RNA, providing analysis of sample size, quantity, and integrity. It uses prefilled "ScreenTape" devices to replace traditional gel electrophoresis, offering results in 1–2 minutes per sample.
Tapestation Assays Available
DNA D1000 (35–1000 bp): 0.1-50ng/u
DNA HS D5000 (100-5000bp): 10-1000pg/ul
RNA: 25-500ng/ul
RNA HS: 500-10,000pg/ul
genomicDNA (200->60,000bp): 5-300ng/ul
Agilent Bioanalyser
The Agilent Bioanalyzer gives a qualitative and semi-quantitative assessment of RNA integrity. It can also be used to examine DNA and proteins.
Bioanalyser Assays Available
DNA 1000 (0.1-50ng/ul)
RNA Nano (5-500ng/ul)
Qubit
We offer Qubit DNA and RNA measurements as a service on request.
The Qubit instrument is also available for users who have their own reagents.
Nanodrop
The NanoDrop Spectrophotometer will read absorbance spectra from 230nm - 600nm giving a quantitative assessment of both nucleic acids and proteins from as little as 1µL of sample. No cuvette is necessary.
There are three departmental NanoDrop Spectrophotometers available for use free of charge. Their locations are:
- Lab C030, access to this lab is restricted (Nanodrop 1000)
- Genomics lab, access to this lab is available at all times (NP60)
- Instrument room, D4, access to this lab is available to all times (NP60)