Next Generation Sequencing
Short-Read Library Preparation Service
The Genomics SRL routinely produce Illumina compatible libraries using the following kits, at a per library cost;
- New England Biolabs NEBNext Ultra Express RNA
- New England Biolabs NEBNext Ultra II FS DNA
- 16S Metagenomic Amplicon libraries
We are happy to discuss other library prep protocols on a per project basis.
Sequencing on Illumina MiSeq
The MiSeq platform provides a flexible approach to next generation sequencing;
- Longer read lengths (up to 300bp, paired-end).
- Dual-indexing supported for greater depth multiplexing of samples.
If the run lengths shown here do not suit your needs or you would like any further information or help with your NGS experiment then please contact us. There are other read lengths available.
MiSeq Specs
Number of reads per run (V3 kits) = 20 million.
Nano and micro flow cells available for smaller experiments.
Good compatiabilty for 16S amplicon work.
| Read Length | Total Run Time | Approx. Output |
| 2 x 75bp | ~24hrs | 3Gb |
| 2 x 300bp | ~65hrs | 12Gb |
Sequencing on Illumina NextSeq
The NextSeq platform offers a higher yield. We can offer this as a limited service in-house or outsource if appropriate. The NextSeq has two types of flow cell with run lengths determined by the user (up to 150bp paired-end);
- High-output - approx. 400 million reads/read-pairs per run.
- Mid-output - approx. 130 million reads/read-pairs per run.
If you require any further information (including costs) then please contact us.
Oxford Nanopore Technologies - P2 Solo and MinION
We can assist you with you library preparation and provide sequencing using the ONT P2 Solo or MinION platform. This is a new service and we are happy to hear more about researchers' experimental needs.
P2 Solo
MinIon
Library Prep
Sample Storage
We will endeavour to store remaining sample material or completed libraries for a period of 12 months before disposal.
Data Storage
We will endeavour to store data for a period of 12 months before deletion.