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Andor Revolution with TIRF

The Andor Revolution XDi spinning disk confocal is designed for fast, optically-sectioned imaging of live samples. The system comes with two cameras and CO2 and temperature incubation, making it ideal for imaging dynamic processes in live cells. Additionally, this system has TIRF functionality, used to image events occurring < 100 nm from the coverslip.

Components

Objectives 100x oil TIRF, 100x oil, 60x oil, 20x air
Lasers 405 nm, 488 nm, 515 nm, 561 nm, 640 nm
FRAP 405 nm, 488 nm, 515 nm, 561 nm, 640 nm
Cameras 2x Andor iXon Ultra and 1x Andor Zyla
Stage motorised xyz
Incubation 37 degrees and CO2
Filters Quad, DAPI, GFP, mCherry, Cy5
Autofocus Hardware-based autofocus

Andor TuCam w/ TIRF