Methods reporting
| Microscope (Make, Model) | Andor Revolution XDi with TIRF |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Nikon, 100x, 1.49, oil, CFI Plan Apo TIRF Nikon, 100x, 1.4, oil, CFI Plan Apo Nikon, 60x, 1.4, oil, CFI Plan Apo Nikon, 20x, 0.75, air, CFI Plan Apo VC |
| Light source(s) |
Select the one/s used: 405 nm, 488 nm, 515 nm, 561 nm, 647 nm lasers |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Spinning disk: Dichroic: 405/488/561/640 nm Secondary dual-camera dichroic: 560 nm Emission filters: DAPI - 460/60 GFP - 525/50 mCherry - 617/73 Cy5 - 685/40 IFP - 708/75 Quad - 440/521/607/700 TIRF: Dichroics: 488/568 and 405/488/561/635 Emission filters: DAPI: 452/45 GFP: 525/50 mCherry: 617/73 Cy5: 685/40 Quad: 440/521/607/700 Information on other filters are available on request. |
| fpbase.org channel set-up | https://www.fpbase.org/microscope/723uDSErj59u2wSxZ5F2f/# |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Yokogawa CSU X-10 spinning disk unit with Borealis Nikon Perfect Focus autofocus Okolab microscope incubator Prior motorised xy stage Prior 400 um z-piezo Andor FRAPPA photomanipulation unit Andor TuCam dual-camera adaptor Nikon motorised TIRF unit |
| Acquisition software and settings | Micro-Manager |
| Camera manufacturer, model | 2x Andor iXon Ultra 897, 1x Andor Zyla |