Methods reporting
| Microscope (Make, Model) | Andor, Revolution XD |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Nikon, 100x, 1.45, oil, Nikon, 40x, 1.30, oil, Nikon, 20x, 0.75, air, Nikon, 10x, 0.45, air, |
| Light source(s) |
Select the one/s used: 457 nm, 488 nm, 515 nm, 561 nm lasers |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Dichroic: 457/515/647 nm or 488/568 nm Secondary dichroic (when using dual-camera): 561 nm longpass Emission filters: CFP - 480/40 GFP - 525/50 YFP - 545/40 RFP - 512/630 GFP TuCam - 514/30 RFP TuCam - 569 LP Information on other filters are available on request. |
| fpbase.org channel set-up | https://www.fpbase.org/microscope/wWHhJCFhLPJQ7Vg5j3hdsL/# |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Nikon Ti-Eclipse Yokogawa CSU X-10 spinning disk unit w/ Borealis Andor FRAPPA photomanipulation unit Andor MicroPoint laser ablation system Okolabs microscope incubator ASI motorised xy stage ASI 400 um z-piezo 2x magnification |
| Acquisition software and settings | Andor iQ |
| Camera manufacturer, model | 2 x Andor iXon Ultra 888 |