BioAFM

The BioAFM is a fluorescence microscope from Nikon combined with atomic force microscopy scan heads from Bruker. The BioAFM allows fast nm-scale topography measurement and mechanical measurements of live biological samples in liquid medium while simultaneously performing fluorescence microscopy.

The fluorescence microscope combines a point scanning confocal with a dual TIRF mode. The AX R confocal has a large field of view of 25 mm, resonant/galvano scan heads, and GaAsPs/PMTs as well as NPSARC. The NSPARC allows 100 nm super resolution and low-noise/sensitive imaging. Single or dual TIRF with photometrics prime 95B sCMOS cameras is also available. This an ideal system for imaging of cellular tissues, cells and subcellular components while measuring topography and mechanical forces.

Located in MCBB room L1.11

Components

AFM scan heads	CellHesion 300: z-range (pull) = 100 μm. NanoWizard V: Scan region of 100x100 μm and z-range (pull) = 15 μm. (Fast Scanner).
Objectives	10x air, 20x air, 40x oil, 60x oil, 100x oil, 100x TIRF oil
Lasers AX	405 nm, 488 nm, 561nm, 640 nm
Lasers eTIRF	488 nm, 561nm, 640 nm
FRAP AX	405 nm, 488 nm, 561nm, 640 nm
Cameras eTIRF	2x Photometrics 95B Prime sCMOS
Detector AX	Tuneable DUXVB detector unit: 3 x GaAsPs + 1x multi alkali PMT - Nikon Spatial ARray Confocal (NSPARC)
Scanner AX	Resonant and Galvano scan heads
Stage	Bruker stage xy and objective lens piezo (200 μm)
Incubation	37 degrees. CO₂ with BioAFM sample holders
Bruker sample holders	Standard for microscopic slide - PetriDishHeater PDH - BioCell
Filters	Bright Field only
Autofocus	Hardware-based autofocus PFS

Please acknowledge the below in your publications and presentations, in addition to following the usual CAMDU acknowledgment policy.

BBSRC Equip – BBSRC Alert 2022

Organisation: University of Warwick

Award Holder: Masanori Mishima

Award Title: BioAFM: Integrated atomic force and light microscopy for mechanochemical cell biology

BBSRC reference: BB/X019934/1