BioAFM
The BioAFM is a fluorescence microscope from Nikon combined with atomic force microscopy scan heads from Bruker. The BioAFM allows fast nm-scale topography measurement and mechanical measurements of live biological samples in liquid medium while simultaneously performing fluorescence microscopy.
The fluorescence microscope combines a point scanning confocal with a dual TIRF mode. The AX R confocal has a large field of view of 25 mm, resonant/galvano scan heads, and GaAsPs/PMTs as well as NPSARC. The NSPARC allows 100 nm super resolution and low-noise/sensitive imaging. Single or dual TIRF with photometrics prime 95B sCMOS cameras is also available. This an ideal system for imaging of cellular tissues, cells and subcellular components while measuring topography and mechanical forces.
Located in MCBB room L1.11.
Hardware details for methods reporting in publications.
Please acknowledge the funding below in your publications and presentations, in addition to following the usual CAMDU acknowledgment policy.
BBSRC Equip – BBSRC Alert 2022
Organisation: University of Warwick
Award Holder: Masanori Mishima
Award Title: BioAFM: Integrated atomic force and light microscopy for mechanochemical cell biology
BBSRC reference: BB/X019934/1
Components
| AFM scan heads |
|
| Objectives | 10x air, 20x air, 40x oil, 60x oil, 100x oil, 100x TIRF oil |
| Lasers AX | 405 nm, 488 nm, 561nm, 640 nm |
| Lasers eTIRF |
488 nm, 561nm, 640 nm |
| FRAP AX | 405 nm, 488 nm, 561nm, 640 nm |
| Detectors AX |
|
| Scanners AX | Resonant and Galvano scan heads |
| Cameras | 2x Photometrics 95B Prime sCMOS |
| Stage | Bruker stage xy and objective lens piezo (200 μm) |
| Incubation | 37°C and CO2 with BioAFM sample holders |
|
Sample holder |
Bruker Standard for microscopic slide - PetriDishHeater PDH - BioCell |
| Filters | Bright Field only |
| Autofocus | Hardware-based autofocus Nikon Perfect Focus System |
