BioAFM
The BioAFM is a fluorescence microscope from Nikon combined with atomic force microscopy scan heads from Bruker. The BioAFM allows fast nm-scale topography measurement and mechanical measurements of live biological samples in liquid medium while simultaneously performing fluorescence microscopy. The fluorescence microscope combines a point scanning confocal with a dual TIRF mode. The AX/AX R confocal has a large field of view of 25 mm and resonant and galvano scan heads with 2 different detectors: a tuneable detector and the NPSARC. The resonant scanner high speed reduces pixel dwell time and therefore photodamage. The single pinhole and the detectors ensure high quality imaging at depths of over 200 μm. The NSPARC, a single photon detector allows 100 nm super resolution and low noise sensitive imaging. Single or dual TIRF with photometrics prime 95B sCMOS cameras is also available. This an ideal system for gentle imaging of cellular tissues, cells and subcellular components while measuring topography and mechanical forces.
Located in MCBB room L1.11
Components
| AFM scan heads | CellHesion 300: z-range (pull) = 100 μm. NanoWizard V: Scan region of 100x100 μm2 and z-range (pull) = 15 μm. (Fast Scanner). |
| Objectives | 10x air, 20x air, 40x oil, 60x oil, 100x oil, 100x TIRF oil |
| Lasers AX | 405 nm, 488 nm, 561nm, 640 nm |
| Lasers eTIRF | 488 nm, 561nm, 640 nm |
| FRAP AX | 405 nm, 488 nm, 561nm, 640 nm |
| Cameras eTIRF | 2x Photometrics 95B Prime sCMOS |
| Detector AX | Tuneable DUXVB detector unit: 3 x GaAsPs + 1x multi alkali PMT - Nikon Spatial ARray Confocal (NSPARC) |
| Scanner AX | Resonant and Galvano scan heads |
| Stage | Bruker stage xy and objective lens piezo (200 μm) |
| Incubation | 37 degrees. CO2 with BioAFM sample holders |
| Bruker sample holders |
Standard for microscopic slide - PetriDishHeater PDH - BioCell |
| Filters | Bright Field only |
| Autofocus | Hardware-based autofocus PFS |