diSPIM microscope

3i's diSPIM microscope, otherwise known as the Marianas LightSheet.

Please contact CAMDU if you would like to use this microscope.

More information on 3i's website here and the dispim.org website here.

Objectives:

• 10x 0.3 NA with a maximum field of view of about 1.49 mm and working distance of 3.5mm
• 40x 0.8 NA with a maximum field of view of about 0.37 mm and working distance of 3.5mm

Filters on the widefield lower objective

Filters on the widefield lower objective are listed below but also on the fpbase.org website here.

Semrock brightline Cy5-4040C-ZHE-ZERO

FF01-628/40-25

FF01-692/40-25

FF660-Di02-25x36 this is correct

Semrock TXRED-4040C-ZHE-ZERO.

624/40

562/40

593 di03

Semrock GFP-3035D-ZHE-ZERO

Semrock DAPI-1160B-ZHE-ZERO

More info here:

https://abnova.com/images/content/product/CytoView_Cy5_Filter_Spec.pdf

https://www.idex-hs.com/store/product-detail/gfp_3035d_zhe_zero/s-000272?search=true

https://www.abnova.com/images/content/product/CytoView_Hoechst33342_Filter_Spec.pdf