FV3000
The FV3000 by Evident/Olympus is a point-scanning confocal with superior optical sectioning capabilites compared to widefield or spinning disk microscopes making it an excellent choice for imaging thicker tissue, particularly fixed samples. It may provide better maximum resolution compared to some spinning disk microscopes although this is limited by the slightly low numerical aperture of the 60x objective.
Simplified Manual available here.Link opens in a new window
Located in the basement of IBRB in room IBRB01.The .oir files open in Fiji (not ImageJ) by just drag dropping, but sometimes there are problems and you have to install a separate plugin, see here for installation instructions.
Please acknowledge the below funding in your publications, in addition to following the usual CAMDU acknowledgment policy.
BBSRC BB/T018070/1
Components
| Objectives | 60x oil, 40x oil, 30x silicone, 20x air, 10x air |
| Lasers | 405 nm, 488 nm, 561nm, 640 nm. Coherent OBIS 20-50mW |
| FRAP | 405 nm, 488 nm, 561nm, 640 nm |
| Detectors | 4x PMTs, and one transmitted detector |
| Stage | Motorised xyz |
| Incubation | Up to 37oC and CO2 (contact CAMDU if you want to use CO2) |
| Filters | Variable bandwidth filters |
| Autofocus | Hardware based |
