MPE methods reporting
| Microscope (Make, Model) | Olympus FVMPE-RS |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Olympus 60x, 1.3, silicone, Super Apochromat (SAPO) Olympus, 60x, 1.42, oil, UPlanXApo Olympus, 30x, 1.05, silicone, UPlanSApo IR Olympus, 20x, 0.5, UPlanFLN Olympus, 10x, 0.3, UPlanFLN |
| Light source(s) |
Spectra-Physics InSight X3 - 690-1300 nm tuneable range - <120 fs pulse width MaiTai HP DS-OL 690-1040 nm tunable range - 690-1040 nm tunable range - <100 fs pulse width |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Beam combiners: Filter sets: FV30-FVG (em 410-455, 495-540, DM 495) FV30-FCY (em 460-500, 520-560, DM 505) FV30-FGR (em 495-540, 575-645, DM 570) FV30-FRCY5 (em 575-645, 660-750, DM 650) SHG/GFP (em 397-412, 500-540, DM 495) NADH (em 420-500, 515, 560, DM 505) |
| fpbase.org channel set-up | not yet available |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Olympus IX83 Galvanometric scanner hybrid resonant-Galvo scanner CellVIVO incubator with CO2 enrichment Olympus motorised stage Mad City Labs piezo insert SIM scanner photomanipulation system Olympus TruFocus zero drift compensation system PicoQuant LSM FLIM upgrade kit - MultiHarp 150 TCSPC module - 2x PMA Hybrid 40 mod detectors |
| Acquisition software and settings |
FluoView SymPhoTime 64 |
| Detector manufacturer, model |
2x GaAsP non-descanned detectors 2x Multialkali non-descanned detectors |