MPE methods reporting
| Microscope (Make, Model) | Olympus FVMPE-RS |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Olympus 60x, 1.3 NA, 0.3mm WD, silicone, Super Apochromat (SAPO) Olympus, 60x, 1.42 NA, 0.15mm WD, oil, UPlanXApo Olympus, 30x, 1.05 NA, 0.8mm WD, silicone, UPlanSApo IR Olympus, 20x, 0.5 NA, 2.1mm WD, UPlanFLN Olympus, 10x, 0.3 NA, 10mm WD, UPlanFLN |
| Light source(s) |
Spectra-Physics InSight X3 - 690-1300 nm tuneable range - <120 fs pulse width MaiTai HP DS-OL 690-1040 nm tunable range - 690-1040 nm tunable range - <100 fs pulse width |
| Filters and dichroic mirrors |
Filter sets: FV30-FVG (em 410-455, 495-540, DM 495) FV30-FCY (em 460-500, 520-560, DM 505) FV30-FGR (em 495-540, 575-645, DM 570) FV30-FRCY5 (em 575-645, 660-750, DM 650) SHG/GFP (em 397-412, 500-540, DM 495) NADH (em 420-500, 515, 560, DM 505) Filters for FLIM detectors: Currently in microscope is a 488 dichroic between the FLIM detectors with 693/46 and 440/40 "cleanup filters". Also available are 483/40 and 550/49. |
| fpbase.org channel set-up | not yet available |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Olympus IX83 Galvanometric scanner hybrid resonant-Galvo scanner CellVIVO incubator with CO2 enrichment Olympus motorised stage Mad City Labs piezo insert Simultaneous scanner photomanipulation system Olympus TruFocus zero drift compensation system PicoQuant LSM FLIM upgrade kit - MultiHarp 150 TCSPC module - 2x PMA Hybrid 40 mod detectors |
| Acquisition software and settings |
FluoView SymPhoTime 64 |
| Detector manufacturer, model |
2x GaAsP non-descanned detectors 2x Multialkali non-descanned detectors |