Methods reporting
| Microscope (Make, Model) | Nikon Ti-Eclipse |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Nikon, 100x, 1.49, oil, CFI Apo TIRF Nikon, 40x, 0.6, air, CFI Plan Apo Nikon, 20x, 0.45, air, CFI Plan Fluor Nikon, 10x, 0.5, air, CFI Plan Fluor |
| Light source(s) |
Nikon Intensilight mercury lamp |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Filter sets: DAPI: ex 360/40, dm 400, ex 460/50 GFP: ex 480/30, dm 505, ex 535/40 mCherry: ex 560/40, dm 585, 630/75 |
| fpbase.org channel set-up | https://www.fpbase.org/microscope/LHThv5UbcxuJQkCcbHQi4D/ |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Nikon Perfect Focus autofocus Nikon motorised xy stage Nikon 100 um z-piezo |
| Acquisition software and settings | NIS-Elements, Micromanager |
| Camera manufacturer, model | Andor iXon 3 |