Methods reporting
| Microscope (Make, Model) | Nikon CSU-W1 spinning disk with SoRa upgrade |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Nikon, 100x, 1.49, oil, CFI SR HP Apo TIRF Nikon, 63x, 1.40, oil, CFI Plan Apo Nikon, 40x, 1.30, oil, CFI Plan Fluor Nikon, 40x, 0.6, air, S Plan Fluor ELWD DIC N1 Nikon, 20x, 0.5, air, CFI Plan Fluor |
| Light source(s) |
Select the one/s used: 405 nm, 488 nm, 561 nm, 638 nm lasers |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Dichroic: 405/488/561/640 nm Secondary dichroic (when using dual-camera): 561 nm LP Emission filters: Blue - 446/60 Green - 525/50 Red - 600/52 FRed - 708/75 Information on other filters are available on request. |
| fpbase.org channel set-up | https://www.fpbase.org/microscope/ZMBg8xKHJmynSD33m8RuFj/# |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Yokogawa CSU-W1 spinning disk unit with 50 um and SoRa disks Nikon Perfect Focus autofocus Okolab microscope incubator Nikon motorised xy stage Nikon 200 um z-piezo Bruker MiniScanner photomanipulation unit |
| Acquisition software and settings | NIS-Elements |
| Camera manufacturer, model | 2 x Photometrics 95B Prime |