Nikon Widefield Ti2-E (NW1)
NW1 is a widefield microscope from Nikon. NW1 is optimised for live-cell, multi-colour fluorescence experiments and, combined with the in-built autofocus, is particularly suited to overnight timelapse imaging of cells and sub-cellular components. Its large of Field Of View of 25 mm enables the capture of more data in a single image with a fast acquisition rate thanks to its Kinetix camera. Multipositions, z-stack and tiling are easy to setup.
The software NIS Elements has a deconvolution functionality as well as other analysis tools (GA3 Analysis and JOBS available on Dolly).
Located in MCBB room L1.23.
Hardware details for methods reporting in publications.
Components
| Objectives | 100x oil, 40x oil, 20x air, 10x air, 4x air |
| LEDs | 378/52 nm, 474/27 nm, 554/23 nm, 635/18 nm |
| Cameras | Photometrics Kinetix (88 fps in 12-bit mode) |
| Filters | DAPI, FITC, GFP, TRITC, DsRed2 and Cy5 |
| Stage | motorised xyz |
| Autofocus | Hardware-based autofocus - Perfect Focus System |
| Incubation | 37 degrees, CO2 |
| Sample holder | 2 x 35 mm dishes + 1 microscopic slide ; Multiwell plate |
| Accessories | Micromanipulator can be installed on request (McAinsh lab) |
