Nikon Widefield Ti2-E (NW2)
NW2 is a widefield microscope from Nikon. NW2 is optimised for live-cell, multi-colour fluorescence experiments and, combined with the in-built autofocus, is particularly suited to overnight timelapse imaging of cells and sub-cellular components. Its large of Field Of View of 25 mm enables the capture of more data in a single image with a fast acquisition rate thanks to its Kinetix camera. Multipositions, z-stack and tiling are easy to setup.
The software NIS Elements has a deconvolution functionality as well as other analysis tools (GA3 Analysis and JOBS available on Dolly).
NW2 has a Photostimulation device, the OptiMicroscan to perform photoactivation, optogenetic and FRAP experiments and is equipped with a black incubation chamber.
Located in MCBB room L1.11.
Hardware details for methods reporting in publications.
Components
| Objectives | 100x oil, 40x oil, 20x air, 10x air, 4x air |
| LEDs | 378/52 nm, 474/27 nm, 554/23 nm, 635/18 nm |
| Cameras | Photometrics Kinetix (88 fps in 12-bit mode) |
| Filters | DAPI, FITC, GFP, TRITC, DsRed2 and Cy5 |
| Stage | motorised xyz |
| Autofocus | Hardware-based autofocus - Perfect Focus System |
| Incubation | 37 degrees, CO2, black incubation chamber. |
| Sample holder | 2 x 35 mm dishes + 1 microscopic slide ; Multiwell plate |
| Photostimulation | 405 nm, 445 nm, 505 nm, 660 nm. OptiMicroscan (OMS). |
