NW2 methods reporting

Microscope (Make, Model)	Nikon, ECLIPSE Ti2-E
Inverted or upright	Inverted
Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any), working distance	Select the one/s used: Nikon, 100x, 1.45, oil, CFI Plan Apochromat λD Nikon, 40x, 1.3, oil, CFI Plan Fluor λD Nikon, 20x, 0.8, air, CFI Plan Apochromat λD Nikon, 10x, 0.45, air, CFI Plan Apochromat λD Nikon, 4x, 0.2, air, CFI Plan Apochromat λD
Light source(s)	CoolLED pE4000 Excitation wavelengths, select the one/s used: Quad cube: 4x Single Exciters: 378/52; 474/27; 554/23; 635/18 nm inside the CoolLED with following excitation wavelengths set at : DAPI: 365 nm (fixed sample) , can be changed to 385 nm (live sample) FITC: 470 nm TRITC: 550 nm CY-5: 635 nm Dual cube : ZET488/561x (combined with Quad Single Exciters). GFP: 490 nm (479-487.5 nm) DsRed2: 550 nm (553-566 nm)
Filters, dichroic mirrors, beam splitters	Select the one/s used: More filter information available on request. Quad: Semrock LED-DA/FI/TR/Cy5-4X-B-000. Dichroic: 409/493/575/652. Emission filters: 432/515/595/730-25 DAPI: 432 nm (414-450 nm) FITC: 515 nm (499.5-530 nm) TRITC: 595 nm (580-611 nm) CY-5: 730 nm (661-800 nm) Dual: Chroma 59904. Beam splitter: ZT488/561rpc Emission filters: ZET488/561m GFP: 523 nm (502-545 nm) DsRed2: 641 nm (575-707 nm)
Spectra	NW1:Nikon Widefield 1 on FPBaseLink opens in a new window Dual cube with Quad single exciters: https://www.chroma.com/spectra-viewer?share=b9036cc2-2d72-4baa-9aab-13005cc25c2aLink opens in a new window
Camera manufacturer, model	Photometrics Kinetix
Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective	5% CO₂ Okolab Digital CO2-UNIT-BL with Air pump. 37.0°C Okolab Black incubation chamber H203. Motorised xy stage Mad CityLabs MLC 200 um z-piezo Okolab Sample Holder for stage top incubator: GS35-INSERT: 2x 35 mm dishes + 1 microscopic slide ; OR Multiwell plate (no need extra adapter).
Acquisition software and settings	NIS Elements v6.10.02