UltraVIEW methods reporting
| Microscope (Make, Model) | PerkinElmer, UltraVIEW VoX |
| Inverted or upright | Inverted |
| Objective: manufacturer, magnification, numerical aperture, immersion, correction (if any) |
Select the one/s used: Nikon, 100x, 1.40, oil, Plan Apo VC Nikon, 60x, 1.40, oil, Plan Apo VC Nikon, 40x, 1.30, oil, Plan Fluor Nikon, 20x, 0.75, air, Plan Apo VC |
| Light source(s) |
Select the one/s used: 405 nm, 488 nm, 561 nm, 640 nm lasers |
| Filters, dichroic mirrors, beam splitters |
Select the one/s used: Dichroic: 405/488/561/640 nm Secondary dichroic (when using dual-camera): 580 nm longpass Emission filters, wheel 1 (primary camera): 488 nm for imaging - 525/50 405 and 561 nm for imaging - 425/60, 615/70 640 nm for imaging - 485/60, 705/90 Emission filters, wheel 2 (secondary camera): 590 LP Information on other filters are available on request. |
| fpbase.org channel set-up | https://www.fpbase.org/microscope/2dfmVdwAV7iCwrDouf653J/ |
| Additional hardware used, e.g., stage motor, incubation chamber and set-up, Piezo stage or objective |
Yokogawa CSU X-10 spinning disk unit Solent Scientific microscope incubator Prior motorised xy stage Prior 400 um z-piezo |
| Acquisition software and settings | Volocity v6.3.1 |
| Camera manufacturer, model | 1x SCMOs Hamamatsu Orca-Flash4.0 v3 (installed November 2022) 2 x Hamamatsu Orca-R2 interline CCD (old one) |