Microsystems are key enabling technologies, with applications found in almost every industrial field, including in vitro diagnostic, energy harvesting, automotive, telecommunication, drug screening. Yet, their development for commercial or educational purposes has typically been limited to specialized laboratories in upper-income countries. In this paper, we describe a range of low-cost approaches and equipment (below £1000), which will enable researchers to build a low-cost toolbox.
Review on how stress increases the susceptibility to herpes simplex virus type 1 primary and recurrent infections
Following primary infection, HSV-1 establishes latency and reactivates under stress. Stress disturbs inner immune Yin–Yang balance, increasing the susceptibility to HSV-1 infection. In latent infection, stress induces changes in hormone level, chromosomal modification and oxidation, thereby disturbing the Yin–Yang balance between HSV-1 and the host defense, leading to recurrent diseases.
Co-lead author Swati A. Kumar in a collaboration with Annabelle Ballesta, Alper Okyar, Francis Lévi, Enza Piccolo and us entitled: Sex-, feeding-, and circadian time-dependency of P-glycoprotein expression and activity - implications for mechanistic pharmacokinetics modeling, we described how time-of-day of drug administration might lead to different pharmacokinetic profiles in male and female mice.
Alper Okyar, Swati Kumar, Elisabeth Filipski, Enza Piccolo, Narin Ozturk, Helena Xandri-Monje, Zeliha Pala, Kristin Abraham, Ana Rita Gato de Jesus Gomes, Mehmet N. Orman, Xiao-Mei Li, Robert Dallmann, Francis Lévi, Annabelle Ballesta, Scientific Reports, 2019, DOI: 10.1038/s41598-019-46977-0
Hyperbranched poly(ethylenimine-co-oxazoline) by thiol-yne chemistry for non-viral gene delivery:investigating the role of polymer architecture
Happy to have been able to help out for this interesting new paper looking into new ways of transfecting cells.
RAFT Emulsion Polymerization as a Platform to Generate Well‐Defined Biocompatible Latex Nanoparticles
The first paper in collaboration with the group of Prof. Seb Perrier (Warwick Chemistry)
Current approaches to generate core–shell nanoparticles for biomedical applications are limited by factors such as synthetic scalability and circula- tory desorption of cytotoxic surfactants. Developments in controlled radical polymerization, particularly in dispersed states, represent a promising method of overcoming these challenges. In this work, well-defined PEGylated nanoparticles are synthesized using reversible addition fragmentation chain transfer emulsion polymerization to control particle size and surface compo- sition and were further characterized with light scattering, electron micros- copy, and size exclusion chromatography. Importantly, the nanoparticles are found to be tolerated both in vitro and in vivo, without the need for any puri- fication after particle synthesis. Pharmacokinetic and biodistribution studies in mice, following intraperitoneal injection of the nanoparticles, reveal a long (>76 h) circulation time and accumulation in the liver.