DV1 is a widefield deconvolution microscope from Applied Precision Instruments (API). DV1 is optimised for live-cell, multi-colour fluorescence experiments and, combined with the in-built autofocus, is particularly suited to overnight timelapse imaging of cells and sub-cellular components. The image acquisition software (SoftWorX) has deconvolution functionality which can be deployed increase contrast and resolution and improve optical sectioning.
|Objectives||100x oil, 60x oil, 40x oil, 20x air|
|LEDs||405 nm, 488 nm, 561nm, 640 nm|
|FRAP||405 nm, 488 nm, 561nm, 640 nm|
|Cameras||Photometrics CoolSNAP HQ interline CCD|
|Incubation||37 degrees, CO2|
|Filters||DAPI, CFP, FITC, GFP, YFP, TRITC, mCherry, Cy5|
Figure 1. Optical performance monitoring by measuring the width of sub-resolution fluorescent beads (not deconvolved). The Rayleigh limit is defined here as the distance between the central peak and the first minimum. Measured for n = 5 beads in x (red), y (blue) and z (green) axes.
Figure 2. Monthly measurement of the fluorescence LED illumination power output at the microscope back aperture. "Wavelength (nm)" is the wavelength at which power is measured.