The UltraVIEW is a dual-camera spinning disk confocal from PerkinElmer. Provides optically sectioned 3D datasets with higher speed and less photobleaching than a standard point-scanning confocal microscope. Suitable for high-speed, multi-colour volumetric fluorescence microscopy.
|Objectives||100x oil, 60x oil, 40x oil, 20x air (on request)|
|Lasers||405 nm, 488 nm, 561nm, 640 nm|
|FRAP||405 nm, 488 nm, 561nm, 640 nm|
|Cameras||2x Hamamatsu Orca-R2 CCD|
|Filters||DAPI, GFP, mCherry, Cy5, 580 nm dichroic for dual-colour|
Figure 1. Optical resolution of the UltraVIEW spinning disk measured monthly using sub-resolution fluorescent beads.
Figure 2. Output power at the back aperture of the UltraVIEW spinning disk measured monthly to monitor performance.