Double Digest Protocol
Greg’s Double Digest Protocol (18.5.09)
Digest at 37°C for 3h
|
|
Insert (PCR Product) |
Vector |
|
ddH2O |
73 |
36.5 |
|
NEB Buffer 3 (10x) |
10 |
5 |
|
BSA (40x) |
2.5 |
1.25 |
|
Template DNA |
10 |
5 |
|
Nde1 Enzyme |
2.5 |
1.25 |
|
BamH1 Enzyme |
2.0 |
1 |
|
|
|
|
|
Total |
100µl |
50µl |
|
|
|
|