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Double Digest Protocol

Greg’s Double Digest Protocol (18.5.09)


Digest at 37°C for 3h



Insert (PCR Product)

Vector

ddH2O

73

36.5

NEB Buffer 3 (10x)

10

5

BSA (40x)

2.5

1.25

Template DNA

10

5

Nde1 Enzyme

2.5

1.25

BamH1 Enzyme

2.0

1




Total

100µl

50µl