Ligation Protocol
Greg’s Ligation Protocol (18.5.09)
Ligate at rt for 2h
|
|
5:1 Ratio |
3:1 Ratio |
|
Quick Ligation Buffer (2x) |
7 |
5 |
|
Cut Insert DNA |
5 |
3 |
|
Cut Vector DNA |
1 |
1 |
|
T4 Ligase |
1 |
1 |
|
|
|
|
|
Total |
14µl |
10µl |
|
|
|
|