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Membrane potential dynamics unveil the promise of Bioelectrical Antimicrobial Susceptibility Testing (BeAST) for anti-fungal screening

Tailise Carolina de Souza-Guerreiro, Letícia Huan Bacellar, Thyerre Santana da Costa, Conor LA Edwards, Ljubica Tasic & Munehiro Asally

Membrane potential is a useful marker for antimicrobial susceptibility testing (AST) due to the fundamental characteristic of vital cells. However, the difficulties associated with measuring the membrane potential in microbes restrict its broad application. In this study, we present Bioelectrical AST (BeAST) using the model fungus Saccharomyces cerevisiae . The results suggest that BeAST holds promise for screening anti-fungal compounds, offering a valuable approach to tackling antimicrobial resistance.

mBio. July 2024