Research Interests
Using a number of potent cytotoxins, including ricin and Shiga-like toxin 1, the group has several main areas of research concerning:
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how these proteins enter mammalian cells
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the steps involved in membrane traversal of the catalytic subunits that enables them to reach the cytosol
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the refolding of toxin and inactivation of ribosomes
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the global response of cells to toxin treatments
- Pietroni, Paola, Vasisht, Nishi, Cook, Jonathan P., Roberts, David M., Lord, Mike, Hartmann-Petersen, Rasmus, Roberts, L. M. (Lynne M.), Spooner, Robert A., 2013. The proteasome cap RPT5/Rpt5p subunit prevents aggregation of unfolded ricin A chain. Biochemical Journal, 453 (3), pp. 435-445
- Lee, Hannah, Ph.D., Sparkes, Imogen, Gattolin, Stefano, Dzimitrowicz, Natasha, Roberts, L. M. (Lynne M.), Hawes, C. R., Frigerio, Lorenzo, 2012. An Arabidopsis reticulon and the atlastin homologue RHD3-like2 act together in shaping the tubular endoplasmic reticulum. New Phytologist, 197 (2), pp. 481-489
- Li, Shuyu, Spooner, Robert A., Hampton, Randolph Y., Lord, Mike, Roberts, L. M. (Lynne M.), 2012. Cytosolic entry of Shiga-like toxin A chain from the yeast endoplasmic reticulum requires catalytically active Hrd1p. PLoS ONE, Vol.7 (No.7)
- Sorieul, Mathias, Langhans, Markus, Guetzoyan, Lucie J., Hillmer, Stefan, Clarkson, Guy J., Lord, Mike, Roberts, L. M. (Lynne M.), Robinson, David G., Spooner, Robert A., Frigerio, Lorenzo, 2011. An Exo2 derivative affects ER and Golgi morphology and vacuolar sorting in a tissue-specific manner in arabidopsis. Traffic, Vol.12 (No.11), pp. 1552-1562
- Pawar, Vidya, De, Antara, Briggs, Laura, Omar, Mahmoud M., Sweeney, Sean T., Lord, Mike, Roberts, L. M. (Lynne M.), Spooner, Robert A., Moffat, Kevin G., 2011. RNAi screening of Drosophila (Sophophora) melanogaster S2 cells for ricin sensitivity and resistance. Journal of Biomolecular Screening, Vol.16 (No.4), pp. 436-442
- Marshall, Richard S., Frigerio, Lorenzo, Roberts, L. M. (Lynne M.), 2010. Disulfide formation in plant storage vacuoles permits assembly of a multimeric lectin. Biochemical Journal, Vol.427 (No.Part 3), pp. 513-521
- Guetzoyan, Lucie J., Spooner, Robert A., Boal, Frédéric, Stephens, David J., Lord, Mike, Roberts, L. M. (Lynne M.), Clarkson, Guy J., 2010. Fine tuning Exo2, a small molecule inhibitor of secretion and retrograde trafficking pathways in mammalian cells. Molecular BioSystems, Vol.6 (No.10), pp. 2030-2038
- Guetzoyan, Lucie J., Spooner, Robert A., Lord, Mike, Roberts, L. M. (Lynne M.), Clarkson, Guy J., 2010. Simple oxidation of pyrimidinylhydrazones to triazolopyrimidines and their inhibition of Shiga toxin trafficking. European Journal of Medicinal Chemistry, Vol.45 (No.1), pp. 275-283
- Li, Shuyu, Spooner, Robert A., Allen, Stuart C. H., Guise, Christopher P., Ladds, Graham, Schnöder, Tina, Schmitt, Manfred J., Lord, Mike, Roberts, L. M. (Lynne M.), 2010. Folding-competent and folding-defective forms of Ricin A chain have different fates following retrotranslocation from the endoplasmic reticulum. Molecular Biology of the Cell, Vol.21 (No.15), pp. 2543-2554
- Boal, Frederic, Guetzoyan, Lucie, Sessions, Richard B., Zeghouf, Mahel, Spooner, Robert A., Lord, Mike (J. Mike), Cherfils, Jacqueline, Clarkson, Guy J., Roberts, L. M. (Lynne M.), Stephens, David J., 2010. LG186: an inhibitor of GBF1 function that causes Golgi disassembly in human and canine cells. Traffic, Vol.11 (No.12), pp. 1537-1551
- Mayerhofer, Peter U., Cook, Jonathan P., Wahlman, Judit, Pinheiro, Teresa J. T., Moore, Katherine A. H., Lord, Mike, Johnson, Arthur E., Roberts, L. M. (Lynne M.), 2009. Ricin A chain insertion into endoplasmic reticulum membranes is triggered by a temperature increase to 37 degrees C. Journal of Biological Chemistry, Vol.284 (No.15), pp. 10232-10242
- Marshall, Richard S., Jolliffe, Nicholas A., Ceriotti, Aldo, Snowden, Christopher J., Lord, Mike, Frigerio, Lorenzo, Roberts, L. M. (Lynne M.), 2009. The role of CDC48 in the retro-translocationof non-ubiquitinated toxin substrates in plant cells. Journal of Biological Chemistry, Vol.283, pp. 15869-15877
- Spooner, Robert A., Watson, Peter, Smith, Daniel C., Boal, Frédéric, Amessou, Mohammed, Mohammed, Ludger, Clarkson, Guy J., Lord, Mike, Stephens, David J., Roberts, L. M. (Lynne M.), 2008. The secretion inhibitor Exo2 perturbs trafficking of Shiga toxin between endosomes and the trans-Golgi network. Biochemical Journal, Vol.414 (No.3), pp. 471-484
- Castilho, Priscila V., Goto, Leandro S., Roberts, L. M. (Lynne M.), Araujo, Ana Paula U., 2008. Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds. FEBS Journal, Vol.275 (No.5), pp. 948-959
- Spooner, Robert A., Harte, Philip J., Cook, Jonathan P., Pietroni, Paola, Rogon, Christian, Höhfeld, Jörg, Roberts, L. M. (Lynne M.), Lord, Mike, 2008. Cytosolic chaperones influence the fate of a toxin dislocated from the endoplasmic reticulum. Proceedings of the National Academy of Sciences of the United States of America, Vol.105 (No.45), pp. 17408-17413
- Chamberlain, Kerry L., Marshall, Richard S., Jolliffe, Nicholas A., Frigerio, Lorenzo, Ceriotti, Aldo, Lord, Mike, Roberts, L. M. (Lynne M.), 2008. Ricin B chain targeted to the endoplasmic reticulum of tobacco protoplasts is degraded by a CDC48- and vacuole-independent mechanism. Journal of Biological Chemistry, Vol.283 (No.48), pp. 33276-33286
- Tolley, Nicholas, Sparkes, Imogen, Hunter, P. R. (Paul R.), Craddock, Christian P., Nuttall, J. (James), Roberts, L. M. (Lynne M.), Hawes, C. R., Pedrazzini, Emanuela, Frigerio, Lorenzo, 2008. Overexpression of a plant reticulon remodels the lumen of the cortical endoplasmic reticulum but does not perturb protein transport. Traffic, Vol.9 (No.1), pp. 94-102
- Allen, Stuart C. H., Moore, Katherine A. H., Marsden, Catherine J., Fülöp, Vilmos, Moffat, Kevin G., Lord, Mike, Ladds, Graham, Roberts, L. M. (Lynne M.), 2007. The isolation and characterisation of temperature-dependent Ricin A chain molecules in Saccharomyces cerevisiae.. FEBS Journal, Vol.274 (No.21), pp. 5586-5599
- Hunter, Paul R., Craddock, Christian P., Di Benedetto, Sara, Roberts, L. M. (Lynne M.), Frigerio, Lorenzo, 2007. Fluorescent reporter proteins for the tonoplast and the vacuolar lumen identify a single vacuolar compartment in Arabidopsis cells. Plant Physiology, Vol.145 (No.4), pp. 1371-1382
- Amessou, Mohamed, Fradagrada, Alexandre, Falguières, Thomas, Lord, Mike, Smith, Daniel C., Roberts, L. M. (Lynne M.), Lamaze, Christophe, Johannes, Ludger, 2007. Syntaxin 16 and syntaxin 5 are required for efficient retrograde transport of several exogenous and endogenous cargo proteins. Journal of Cell Science, Vol.120 (No.8), pp. 1457-1468
- Allen, Stuart C. H., Moore, Katherine A. H., Marsden, Catherine J., Fülöp, Vilmos, Moffat, Kevin G., Lord, Mike, Ladds, Graham, Roberts, L. M. (Lynne M.), 2007. The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae. FEBS JOURNAL, 274 (21), pp. 5586-5599
- Williams, Jonathan P., Smith, Daniel C., Green, Brian N., Marsden, Brian D., Jennings, Keith R., Roberts, L. M. (Lynne M.), Scrivens, James H., 2006. Gas phase characterization of the noncovalent quaternary structure of Cholera toxin and the Cholera toxin B subunit pentamer. Biophysical Journal, Vol.90 (No.9), pp. 3246-3254
- Smith, Daniel C., Sillence, Daniel J., Falguie`res, Thomas, Jarvis, Rosemary M., Johannes, Ludger, Lord, Mike, Platt, Frances M., Roberts, L. M. (Lynne M.), 2006. The association of shiga-like toxin with detergent-resistant membranes is modulated by glucosylceramide and is an essential requirement in the endoplasmic reticulum for a cytotoxic effect. Molecular Biology of the Cell, Vol.17 (No.3), pp. 1375-1387
- Jolliffe, Nicholas A., Di Cola, Alessandra, Marsden, Catherine J., Lord, Mike, Ceriotti, Aldo, Frigerio, Lorenzo, Roberts, L. M. (Lynne M.), 2006. The N-terminal ricin propeptide influences the fate of ricin A-chain in tobacco protoplasts. JOURNAL OF BIOLOGICAL CHEMISTRY, 281 (33), pp. 23377-23385
- Spooner, Robert A., Smith, Daniel C., Easton, A. J. (Andrew J.), Roberts, L. M. (Lynne M.), Lord, Mike, 2006. Retrograde transport pathways utilised by viruses and protein toxins. Virology Journal, Vol.3
- Smith, Daniel C., Spooner, Robert A., Watson, P. D., Murray, J. L., Hodge, T. W., Amessou, Mohamed, Johannes, Ludger, Lord, J.M., Roberts, L. M. (Lynne M.), 2005. Internalized pseudomonas exotoxin A can exploit multiple pathways to reach the endoplasmic reticulum. Traffic, 7 (4), pp. 379-393
- Spooner, Robert A., Watson, Peter, Marsden, Catherine J., Smith, Daniel C., Moore, Katherine A. H., Cook, Jonathan P., Lord, Mike, Roberts, L. M. (Lynne M.), 2004. Protein disulphide-isomerase reduces ricin to its A and B chains in the endoplasmic reticulum. Biochemical Journal, Vol.383 (No.2), pp. 285-293
- Frigerio, Lorenzo, Roberts, L. M. (Lynne M.), 2010. The Synthesis of Ricinus communis Lectins. In Toxic Plant Proteins, Berlin, Heidelberg, Springer, pp. 191-205
| Title | Funder | Award start | Award end |
|---|---|---|---|
| Mechanisms of Target Cell Entry and Applications of Protein Toxins | Wellcome Trust | 01 Mar 2007 | 28 Feb 2012 |
| How are proteins sorted to storage vacuoles in plant seeds? | Leverhulme Trust | 01 Jul 2007 | 30 Jun 2010 |
| The role and impact of endoplasmic reticulum associated degradation in plant cells. | Leverhulme Trust | 01 Mar 2007 | 28 Feb 2010 |
| Co-operative Research for the Development of Vaccines, Adjuvants, Thereapeutics (Cell Responses to and Proteins Exploited by .....) | National Institutes of Health | 01 Aug 2005 | 28 Feb 2010 |
| Cellular Intoxication by Ricin (2) -extension to 11731 RBSLR0009 | Home Office | 01 Jan 2009 | 31 Dec 2009 |
| Selective chemical intervention in membrane trafficking - designing interfacial inhibitors specific to Arf1/Arf=GEF complexes. | BBSRC | 01 Jan 2007 | 31 Dec 2009 |
| Cellular Intoxication by Ricin | Department of Health | 01 Jan 2005 | 31 Dec 2007 |
| Wellcome Prize Studentship | Wellcome Trust | 01 Oct 2002 | 30 Sep 2005 |
| Wellcome Prize Studentship for Kerry Chamberlain | Wellcome Trust | 01 Oct 2002 | 30 Sep 2005 |
| Vacation Bursary - Eilish McCann | BBSRC | 27 Jun 2005 | 19 Aug 2005 |
| Genetic analysis of ricin action | Department of Health | 01 Mar 2004 | 01 Mar 2005 |
| The interaction of ricin in chain with biological membranes | DTI | 01 Jul 2004 | 31 Dec 2004 |
| Real time PCR & high throughput sequencing facilities for biomedical research, microbiology and cell biology | BBSRC | 01 Oct 2003 | 30 Jun 2004 |
| Identifying components of the yeast ERAD pathway and their interaction with toxic substrate | BBSRC | 01 Dec 2003 | 29 Feb 2004 |